The IHC staining of TRAF6 and Ki-67 was performed using VECTASTAIN ABC Detection System (including biotinylated goat anti-rabbit IgG secondary antibody; VECTOR). The whole process was conducted as the manufacturer's instructions. Primary antibodies included rabbit anti-TRAF6 and rabbit anti-Ki-67 (Millipore). The results of TRAF6 and Ki-67 detections were independently evaluated by two neuropathologists. Their LIs were determined according to the percentage of positive cell number to total cell number in 10 randomly selected microscopic fields at × 400.
Vectastain abc detection system
Manufactured by Vector Laboratories
Sourced in United States
The VECTASTAIN ABC Detection System is a sensitive and reliable reagent system used for the detection of antigens in tissue sections. It utilizes an avidin-biotin complex to amplify the signal, resulting in enhanced visualization of target proteins. The system is designed to provide consistent and reproducible results in immunohistochemical staining procedures.
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Lab products found in correlation
2 protocols using vectastain abc detection system
1
Immunohistochemical Analysis of TRAF6 and Ki-67
2
HMGA2 and MMP2 Immunohistochemistry
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HMGA2 and MMP2 were detected by the VECTASTAIN ABC Detection System (VECTOR, Burlingame, USA) following the manufacturer's instructions. Primary antibodies included mouse anti‐HMGA2 diluted at 1:400 (Abcam, Cambridge, USA) and rabbit anti‐MMP2 diluted at 1:100 (CST, Danvers, USA). The IHC staining was analyzed with a Leica DM6000B upright microscope with CCK digital camera (Wetzlar, Germany). The labeling index [LI (%)] was calculated based on the percentage of cell number between positive staining and total.
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