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Irβ sc 711

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IRβ (sc-711) is an antibody product offered by Santa Cruz Biotechnology. It is designed to detect the insulin receptor beta (IRβ) protein in various biological samples. IRβ plays a key role in insulin signaling and glucose metabolism.

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Lab products found in correlation

Ab128568, by Abcam (1 mentions) Igf 1rβ, by Cell Signaling Technology (1 mentions) P irs 1, by Merck Group (1 mentions) Ab54477, by Abcam (1 mentions) Bca assay, by Thermo Fisher Scientific (1 mentions) P erk1 2, by Cell Signaling Technology (1 mentions) Pan akt, by Cell Signaling Technology (1 mentions) P foxo1 3, by Cell Signaling Technology (1 mentions) Fatty acidsynthase, by Abcam (1 mentions) Vinculin, by Merck Group (1 mentions) Foxo1, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Ripa buffer, by Merck Group (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Total akt pkb a2210, by Santa Cruz Biotechnology (1 mentions) Bio 1d software, by Avantor (1 mentions) P jnk thr183 tyr185, by Cell Signaling Technology (1 mentions) P erk thr202 tyr204, by Cell Signaling Technology (1 mentions) Ecl western blotting system, by Thermo Fisher Scientific (1 mentions) Pvdf membranes and protein standards, by Bio-Rad (1 mentions)

Spelling variants of this product

Sc-711 (14 citations) Anti-IRβ (sc-711; (2 citations)

3 protocols using irβ sc 711

1

Insulin Signaling Pathway Protein Analysis

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Tissue fragments and cells were homogenized in RIPA buffer (EMD
Millipore) supplemented with protease and phosphatase inhibitors (Biotool).
Lysates were separated from insoluble material by centrifugation (12,000
RPM, 15 min, 4°C) and total protein content was determined by BCA
assay (ThermoFisher). Equal protein ammounts (~10 μg) were
resolved by SDS-PAGE and transferred to polyvinylidene fluoride (PVDF)
membranes (EMD Millipore). Membranes were immunoblotted with the indicated
antibodies: p-IR/IGF1R (#3024, Cell Signaling), IRβ (sc-711, Santa
Cruz), IGF1Rβ (#3027, Cell Signaling), p-IRS1 (09–432,
Millipore), IRS1 (611394, BD), p-AktS473 (#9271, Cel Signaling),
Akt pan (#4685, Cell Signaling), p-FoxO1/3 (#9464, Cell
Signaling), FoxO1 (#9454, Cell Signaling), p-ERK1/2 (#9101, Cell
Signaling), ERK1/2 (#9102, Cell Signaling), CPT1A (ab128568,
Abcam), HADHA (ab54477, Abcam), SREBP1 (sc-8984, Santa Cruz), Fatty Acid
Synthase (ab22759, Abcam), Vinculin (#3574, Chemicon).
Batista T.M., Garcia-Martin R., Cai W., Konishi M., O’Neill B.T., Sakaguchi M., Kim J.H., Jung D.Y., Kim J.K, & Kahn C.R. (2019). Multi-dimensional Transcriptional Remodeling by Physiological Insulin In vivo. Cell reports, 26(12), 3429-3443.e3.
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2

Immunoblotting Analysis of Insulin Signaling

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Frozen liver and epididymal WAT lysates were prepared in RIPA buffer, as described previously (Delibegovic et al., 2007 (link)). Proteins were separated by 4–12% SDS-PAGE and transferred to nitrocellulose membranes. Immunoblots were performed using antibodies from Cell Signaling Technology (Cell Signaling by NEB, Hitchin, UK) (unless stated otherwise) against phospho-S6 (s235/236) 4858S; total S6 2217S; phospho-Akt/PKB (s473) 9271S; phospho-eIF2α (s51) 9721S; total eIF2α 5324S; total IRE1α 3294S; phospho-IRE1α (s724) PA1-16927 (Thermo Scientific); phospho-IR (tyr1163/1163) 44804G (Invitrogen, Paisley, UK); phospho-IR (tyr1158) 44802G (Invitrogen); IR-β sc-711 (Santa Cruz, Dallas, TX, USA); total Akt/PKB A2210 (Santa Cruz); and extracellular signal-regulated kinase 2 (ERK2) I2908 (Santa Cruz). Proteins were visualized with enhanced chemiluminescence and quantified using Bio-1D software (Peqlab, Sarisbury Green, UK).
Lees E.K., Król E., Grant L., Shearer K., Wyse C., Moncur E., Bykowska A.S., Mody N., Gettys T.W, & Delibegovic M. (2014). Methionine restriction restores a younger metabolic phenotype in adult mice with alterations in fibroblast growth factor 21. Aging Cell, 13(5), 817-827.
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3

Molecular Signaling Pathway Analysis

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Cholesterol and triglyceride (TG) concentrations were determined using kits purchased from Wiener Lab Group (Rosario, Argentina). Free fatty acids (FFA) and insulin levels were determined using kits from Coat-A-Count (Siemens, CA). Antibodies for α-tubulin (sc-23948), and the insulin receptor (IR)β (sc-711) were from Santa Cruz Biotechnology (Santa Cruz, CA). Primary antibodies for extracellular signal-regulated kinases 1/2 (ERK1/2) (#4695), p-ERK (Thr202/Tyr204) (#4370), JNK (#9252), p-JNK (Thr183/Tyr185) (#9251), p-PKCδ (Thr505) (#9374), IKKα (#2682), p-IKKα/β (#2697) (Ser176/180), protein kinase B (AKT) (#4691), p-AKT (Ser473) (#4060), were obtained from Cell Signaling Technology (Danvers, MA). Antibody for p-IR (Tyr1162/Tyr1163) (448046G) was obtained from Invitrogen (Waltham, MA). Antibodies for insulin receptor substrate 1 (IRS1) (06–248), p-IRS1 (Tyr612) (09–432) and PTP1B (ABS40), and Luminata™ HRP chemiluminescence detection reagent were from Millipore Corp. (Billerica, MA). PVDF membranes and protein standards were obtained from BIO-RAD (Hercules, CA). The ECL Western blotting system was from Thermo Fisher Scientific Inc. (Piscataway, NJ). EC and all other reagents were purchased from Sigma (St. Louis, MO).
Cremonini E., Bettaieb A., Haj F.G., Fraga C.G, & Oteiza P.I. (2016). (−)-Epicatechin improves insulin sensitivity in high fat diet-fed mice. Archives of biochemistry and biophysics, 599, 13-21.
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