8 well chambers

Manufactured by Corning

The 8-well chambers are a laboratory equipment product designed for various cell culture and microscopy applications. These chambers provide a controlled environment for culturing cells or conducting experiments. Each chamber has a capacity of 8 individual wells, allowing for parallel experiments or sample preparation.

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Lab products found in correlation

Alexa fluor 488 conjugated anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Corning (1 mentions)

Close spelling variants of this product

8-μm-pore transwell chambers in 24-well plates 24-well Transwell chambers with 8-µm pore membranes Transwell chambers (24-well insert; pore size: 8 µm 24-well transwell chambers with 8 μm-pore 24-well transwell chambers (0.8 μm 24-well Boyden chambers with 8 μm-pore polycarbonate membranes 24-well transwell chambers with 8 μm pore size polycarbonate membrane Transwell chambers (24-well insert; 8-μm pore size; 24-well transwell chambers with 8.0 μm pore size polycarbonate membrane 24-well transwell chambers with 8-µm pore size

3 protocols using 8 well chambers

Quantifying Hypoxia-Induced Syndecan-1 Expression

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HLMECs were grown directly on 8-well chambers (Corning). Cells were then transiently transfected by incubation with 100 nM c-Jun siRNA or scrambled RNA (scRNA) and 2.5 μl/ml Lipofectamine 2000 in antibiotic-free Opti-MEM for 24 h. The medium was then changed to the growth medium, and the cells were cultured for another 48 h prior to assays. Silencing of the respective proteins was validated by Western blot analysis. After being exposed to hypoxia (94% N₂, 1% oxygen, and 5% CO₂) for 18 h, cells were fixed in 4% paraformaldehyde for 15 min and blocked with 2% bovine serum albumin (BSA) in PBS for 1 h at room temperature. Cells were then incubated with anti-syndecan1 antibody (1:100; Cell Signaling) in 1% BSA at 4 °C overnight and followed by incubating with Alexa fluor 488 conjugated anti-mouse IgG (1:200; Invitrogen) in 1% BSA for 2 h at room temperature. The fluorescence intensity was quantified using Quantity One and reported as relative fluorescence units.

Wu F., Wang J.Y., Dorman B., Zeineddin A, & Kozar R.A. (2022). c-Jun-mediated miR-19b expression induces endothelial barrier dysfunction in an in vitro model of hemorrhagic shock. Molecular Medicine, 28, 123.

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Syndecan-1 and Fibrinogen Expression Assay

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HLMECs monolayers were grown directly on 8-well chambers (4 × 10⁴; Corning). After being treated with the test fluids for 6 hours, cells were fixed in 4% paraformaldehyde for 15 minutes and blocked with 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature. Cell were then incubated with anti-syndecan1 antibody or anti-fibrinogen antibody (1:200; Cell Signaling) in 1% BSA at 4°C overnight and followed by incubating with Alexa fluor 488 conjugated anti-rabbit IgG (1:400; Invitrogen) in 1% BSA for one hour at room temperature. The fluorescence intensity was quantified using Quantity One and reported as relative fluorescence units (RFU).

Wu F, & Kozar R.A. (2019). Fibrinogen protects against barrier dysfunction through maintaining cell surface syndecan-1 in-vitro. Shock (Augusta, Ga.), 51(6), 740-744.

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THP-1 Cell Culture and Priming

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THP-1 cells were cultured in Corning® RPMI 1640 media supplemented with 10% fetal bovine serum, namely complete RPMI 1640 (c-RPMI 1640) medium at 5%

{CO}_{2}

and 37°C. Before exposure to MeONPs, THP-1 cells were primed by suspension in c-RPMI 1640 medium containing

1 μ g / mL

PMA and seeded in 96-well plates or 8-well chambers (Corning Inc.) at a density of

3 \times 10^{5} cells / mL

for overnight incubation.

Huang Y., Li X., Xu S., Zheng H., Zhang L., Chen J., Hong H., Kusko R, & Li R. (2020). Quantitative Structure–Activity Relationship Models for Predicting Inflammatory Potential of Metal Oxide Nanoparticles. Environmental Health Perspectives, 128(6), 067010.

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