Anti rad50

Manufactured by Fortis Life Sciences

Sourced in United Kingdom

Anti-RAD50 is a specific antibody used for the detection and quantification of the RAD50 protein. RAD50 is a key component of the MRE11-RAD50-NBS1 (MRN) complex, which is involved in the cellular response to DNA double-strand breaks. The Anti-RAD50 antibody can be used in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the RAD50 protein.

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Lab products found in correlation

Anti smc1, by Fortis Life Sciences (1 mentions) Anti phospho rpa32, by Fortis Life Sciences (1 mentions) Anti phospho p38 mapk, by Proteintech (1 mentions) Anti phospho kap 1, by Fortis Life Sciences (1 mentions) Mouse monoclonal anti gst, by GE Healthcare (1 mentions) Mouse monoclonal anti p53 ps15, by Cell Signaling Technology (1 mentions) Anti actin, by Merck Group (1 mentions) Anti rad51, by Abcam (1 mentions) Anti patm s1981, by Abcam (1 mentions) Anti nbs1, by Fortis Life Sciences (1 mentions) Anti rpa32, by Fortis Life Sciences (1 mentions) Peroxidase affinipure donkey anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions) Anti cldu, by Abcam (1 mentions) Anti prpa32 s33, by Fortis Life Sciences (1 mentions) Anti mre11, by Fortis Life Sciences (1 mentions) Anti brca1, by Proteintech (1 mentions) Anti ctip, by Santa Cruz Biotechnology (1 mentions) Anti flag m2, by Merck Group (1 mentions)

2 protocols using anti rad50

Comprehensive Antibody Panel for DNA Damage Response

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The following antibodies were used in this study: mouse monoclonal anti-γH2A histone family X (H2AX) [#05–636], anti-RPA32 [NA-19 L6], anti-RPA70 [NA-18] and rabbit polyclonal anti-H2B [#07–371] (Merck Millipore); rabbit polyclonal anti-FXR1 [A300-892A], anti-phospho-RPA32 [A300-246A], anti-phospho-SMC1 (S966) [A300-050A], anti-SMC1 [A300-055A], anti-RAD50 [A300-184A] and anti-phospho-KAP1 [A300-767A] (Bethyl Laboratories Inc.); rabbit polyclonal anti-phospho-Chk2 (T68) [#2661], anti-phospho-Chk1 (S317) [#2344], anti-phospho-pRad17 (S645) [#6981], anti-phospho-p38MAPK [#4511] and rabbit polyclonal anti-TOMO20 [11802–1-AP] (Proteintech); mouse monoclonal anti-p53-pS15 [#9286] (Cell Signaling Technology); rabbit polyclonal anti-NBS1 [NB100–143] and anti-MRE11 [NB100–142] (Novus Biologicals); rabbit polyclonal anti-phospho-ATM [ab8192] (Abcam); mouse monoclonal anti-KAP1 [GTX49179], anti-NBS1 [GTX70222], anti-MRE11 [GTX70212] and anti-RAD50 [GTX70282] (GeneTex); mouse monoclonal anti-beta-actin [A53169] (Sigma-Aldrich); mouse monoclonal anti-p53 [sc-126] and anti-ATM [sc-23992] (Santa Cruz); mouse polyclonal anti-RAD51 [70–001] (Bioacademia); and mouse monoclonal anti-GST [27–4577-01] (GE Healthcare).

Qi F., Meng Q., Hayashi I, & Kobayashi J. (2020). FXR1 is a novel MRE11-binding partner and participates in oxidative stress responses. Journal of Radiation Research, 61(3), 368-375.

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Antibody Validation in DNA Damage Response

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The following antibodies were used in this study: anti-FLAG M2 (Sigma-Aldrich, St Louis, USA), anti-RBM14 (Abclonal, Wuhan, China), anti-Actin (Sigma-Aldrich), anti-BRCA1 (Proteintech, Chicago, USA), anti-RPA70 (Abclonal), anti-GST (MBL, Tokyo, Japan), anti-His (MBL), anti-RPA32 (Bethyl, Montgomery, USA), anti-pRPA32 S33 (Bethyl), anti-ATM (Bethyl), anti-pATM S1981 (Abcam, Cambridge, UK), anti-CldU (Abcam), anti-RAD51 (Abcam), anti-MRE11 (Bethyl), anti-RAD50 (Bethyl), anti-NBS1 (Bethyl), anti-CTIP (Santa Cruz Biotech, Santa Cruz, USA), anti-gamma H2AX (CST, Danvers, USA), fluorescein (FITC)-AffiniPure anti-rabbit IgG (H+L) (Jackson ImmunoResearch, Philadelphia, USA), and Alexa Fluorescein 594-AffiniPure anti-mouse IgG (H+L) (Jackson ImmunoResearch), Peroxidase AffiniPure donkey anti-rabbit IgG (H+L) (Jackson ImmunoResearch), and Peroxidase AffiniPure goat anti-mouse lgG (H+L) (Jackson ImmunoResearch).

Li Z., Liao Y., Tang C., Xu L., Peng B, & Xu X. (2023). RBM14 promotes DNA end resection during homologous recombination repair: RBM14 in HR repair. Acta Biochimica et Biophysica Sinica, 55(12), 1864-1873.

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