Rabbit anti human pd l1

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-human PD-L1 is a primary antibody that targets the PD-L1 protein, which is expressed on the surface of various cell types. This antibody can be used for immunohistochemical and flow cytometric applications to detect and analyze PD-L1 expression.

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Lab products found in correlation

Donkey anti goat igg hrp, by Santa Cruz Biotechnology (1 mentions) Anti goat igg hrp, by R&D Systems (1 mentions) Mouse anti human gapdh, by Santa Cruz Biotechnology (1 mentions) Anti rabbit igg hrp, by Cell Signaling Technology (1 mentions) Anti mouse igg hrp, by Cell Signaling Technology (1 mentions) Pe cy7 anti mouse cd8a, by BioLegend (1 mentions) Fitc anti mouse b220, by BioLegend (1 mentions) Pe anti mouse cd45, by BioLegend (1 mentions) Apc anti mouse cd3, by BioLegend (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Alpha naphthoflavone, by Merck Group (1 mentions) Pe anti mouse pd 1, by BioLegend (1 mentions) Invivoplus rat igg2b isotype control, by BioXCell (1 mentions) Goat anti mouse pd l1, by R&D Systems (1 mentions) Anti mouse cd4 apc cy7, by BioLegend (1 mentions) Anti ki67, by Abcam (1 mentions) Anti β actin, by Merck Group (1 mentions) Benzo a pyrene, by Merck Group (1 mentions)

2 protocols using rabbit anti human pd l1

Antibodies for Cell Signaling Analysis

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RB1 (BD Pharmigen, cat. G3-245), Rabbit anti-human RB1 (Cell Signalling Technologies, cat. 9313), Phospho-RB (Thr373) (Thermo Fisher, cat. PA5-64767), Rabbit anti-human/mouse p130 (Cell Signalling Technologies, cat.13610), Rabbit anti-human p107 (Santa Cruz, cat. Sc-318), Rabbit anti-human PD-L1 (Cell Signalling Technologies, cat.13684), Goat anti-mouse PD-L1 (Novus albociclib, cat. AF1019), Rabbit anti-mouse PD-L1 (R&D systems, cat. MAB90781), PVR (Cell Signalling Technologies, cat.13544), mouse anti-human GAPDH (Santa Cruz, cat. Sc-365062), mouse monoclonal anti-human c-myc (9E10, Santa Cruz sc-40; a gift from Dr. Linda Penn), anti-goat IgG-HRP (R&D Systems, cat. HAF109), donkey anti-goat IgG-HRP (Santa Cruz, cat. Sc-2020), anti-rabbit IgG-HRP (Cell Signalling Technologies, cat. 7074), anti-mouse IgG-HRP (Cell Signalling Technologies, cat. 7076). Antibodies were diluted in 5% non-fat dry milk or BSA at dilutions of 1/1000–1/5000.

Shrestha M., Wang D.Y., Ben-David Y, & Zacksenhaus E. (2023). CDK4/6 inhibitors and the pRB-E2F1 axis suppress PVR and PD-L1 expression in triple-negative breast cancer. Oncogenesis, 12(1), 29.

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Comprehensive PD-L1 Detection Protocol

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Antibodies used included rabbit anti-human PD-L1 (#13684, Cell Signaling Technology, Beverly, MA, USA; 1:1000 for Western blot), anti-Cleaved Caspase-3 (#9664, Cell Signaling Technology; 1:250 for IHC), Rabbit anti-human AhR (#83200, Cell Signaling Technology; 1:50 for ChIP), goat anti-mouse PD-L1 (#AF1019, R&D, Minneapolis, MN, USA; 10 µg/mL for IHC), anti-Ki67 (#ab15580, Abcam, Cambridge, MA, USA; 1:500 for IHC), TTF1 (#ab76013, Abcam, Cambridge, MA, USA; 1:200 for IHC), anti-β-Actin (#A1978, Sigma, St. Louis, MO, USA; 1:5000 for WB), APC anti-mouse CD3 (#100235, Biolegend, San Diego, CA, USA; 1:20 for flow cytometry), PE/Cy7 anti-mouse CD8a (#100721, Biolegend; 1:20 for flow cytometry), APC/Cy7 anti-mouse CD4 (#100413, Biolegend; 1:20 for flow cytometry), PE anti-mouse CD45 (#103105, Biolegend; 1:20 for flow cytometry), PE/Cy7 anti-mouse PD-L1 (#124313, Biolegend; 1:20 for flow cytometry), PE anti-mouse PD-1 (#135206, Biolegend; 1:20 for flow cytometry), FITC anti-mouse B220 (#103205, Biolegend, San Diego, CA, USA; 1:20 for flow cytometry), In Vivo Plus anti-mouse PD-L1 (#BP0101), In Vivo Plus Rat IgG 2b Isotype Control (Clone: LTF-2, #BP0090) were purchased from BioXcell (West Lebanon, NH, USA). Benzo(a)pyrene (#B1760) and Alpha-Naphthoflavone (ANF; # N5757) were purchased from Sigma.

Wang G.Z., Zhang L., Zhao X.C., Gao S.H., Qu L.W., Yu H., Fang W.F., Zhou Y.C., Liang F., Zhang C., Huang Y.C., Liu Z., Fu Y.X, & Zhou G.B. (2019). The Aryl hydrocarbon receptor mediates tobacco-induced PD-L1 expression and is associated with response to immunotherapy. Nature Communications, 10, 1125.

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