Anti c1galt1 antibody

One hundred eighty-four tumor specimens collected before chemotherapy were fixed in formalin and embedded in paraffin. The expression of C1GALT1 was evaluated by using an avidin-biotin complex immunoperoxidase staining technique as described previously [37 (link)]. A monoclonal anti-C1GALT1 antibody (Santa Cruz) was used and signals were detected with Super Sensitive Link-Label immunohisto-chemistry Detection System (BioGenex). The specific staining was visualized with 3,3-diaminobenzidine liquid substrate system (Sigma) and counterstained with hematoxylin (Sigma).

Tissue microarray of gastric adenocarcinoma from 111 patients was purchased (HStm-Ade178Sur-01, US Biomax, Inc., MD, USA) for immunohistochemical staining. The tissue microarray was incubated with an anti-C1GALT1 antibody (1:100, Santa Cruz Biotechnology, CA, USA) at 4 °C for 16 h. Super Sensitive^TM Link-Label IHC Detection System (BioGenex, CA, USA) was used and signals were visualized through a 3,3-diaminobenzidine (DAB) liquid substrate system (Sigma, MO, USA). The tissues were counterstained with hematoxylin and mounted with UltraKitt (J.T. Baker, Deventer, Holland). Negative controls were performed through replacing the primary antibody with a control IgG at the same concentration.