Triptic soy agar (Merck, Germany), Columbia agar base (BD, Germany), skimmed milk medium (Difco, USA), Sodium dihydrogen phosphate monohydrate (NaH2PO4.H2O, Merck, Germany), Sodium phosphate dibasic anhydrous (Na2HPO4, Merck, Germany), Sodium azide (NaN3, Merck, Germany), and urea (Merck, Germany) were purchased from the company stated. Microorganism: Escherichia coli ATCC 25922 (E. coli), K. pneumoniae ATCC 700603 and P. mirabilis ATCC 12453 were obtained from Erciyes University, Faculty of Pharmacy, Pharmaceutical Microbiology research laboratory ATCC culture collection. P. mirabilis and K. pneumoniae were stored in skim milk medium at -20 °C and regenerated prior to the experiments. The optical density was determined by spectrophotometer (Azureao, Azure Biosystems, Inc.).
Sodium dihydrogen phosphate monohydrate nah2po4 h2o
Manufactured by Merck Group
Sourced in United States
Sodium dihydrogen phosphate monohydrate (NaH2PO4.H2O) is a chemical compound commonly used in laboratory settings. It is a crystalline solid that serves as a source of phosphate ions and a buffering agent. The compound has a molecular formula of NaH2PO4·H2O, and it is soluble in water.
Automatically generated - may contain errors
Lab products found in correlation
Sodium azide nan3, by Merck Group (2 mentions)
Urea, by Merck Group (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide edc, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Sodium nitrate nano3, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Xradia 520 versa 3d x ray microscope, by Zeiss (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Advanced dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Potassium hydroxide koh pellets, by Merck Group (1 mentions)
5 protocols using sodium dihydrogen phosphate monohydrate nah2po4 h2o
1
Antimicrobial Susceptibility Testing Protocol
2
Fluorescence Labeling of Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The GA sample was obtained from C.E. Roeper GmbH (Hamburg, Germany). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and 7-methoxycoumarin-3-carboxylic acid (MC) were purchased from Invitrogen (Lidingö, Sweden), and were used for fluorescence-labeling of the proteinaceous material in GA and bovine serum albumin (BSA). The BSA was purchased from Sigma-Aldrich (St. Louis, MO, USA). The dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich (Darmstadt, Germany). Di-sodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O) and sodium dihydrogen phosphate monohydrate (NaH2PO4·H2O) were purchased from Merck KGaA (Darmstadt, Germany), and were used for the preparation of 10 mM phosphate buffer at pH 7, 9, and 11. Sodium nitrate (NaNO3) and sodium azide (NaN3) were obtained from Merck KGaA and were used for the preparation of carrier liquid for AF4 analyses. All aqueous solutions were prepared in Milli-Q water (18.2 MΩ/cm) produced by a Milli-Q plus purification system from Millipore Co. Ltd. (Billerica, MA, USA).
3
3D Imaging of Medaka Fish using X-ray Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For microfocus- and synchrotron-based X-ray tomographic microscopy, medaka specimens were fixed in 4% paraformaldehyde (Sigma), 1% glutaraldehyde (Sigma), 0.02 M sodium dihydrogen phosphate monohydrate (NaH2PO4.H2O; Merck) and 0.08 M sodium phosphate dibasic heptahydrate (Na2HPO4.7H2O; Merck) at room temperature overnight. Fixed specimens were stored in 70% ethanol and whole specimens were imaged using the Zeiss Xradia 520 Versa 3D X-ray microscope at Naturalis Biodiversity Center, Leiden, The Netherlands. Specimens were mounted in agarose and scanned at 40kV over 180° with 801 to 1601 projections. Exposure times ranging from 8 to 13 s resulted in a voxel size of 4.82 to 48.18 μm. Synchrotron radiation X-ray tomographic microscopy (SRXTM) was used for regions of interest of critical point dried specimens at the TOMCAT beamline, Swiss Light Source (SLS), Paul Scherrer Institut (PSI), Villigen, Switzerland. Acquisition of scans with 1501 projections over 180° using a 10x objective resulted in a voxel size of 0.65 μm. Energy settings ranging from 12 to 15 keV with exposure timings of 220 to 350 ms were used. Volume renderings and images of tomographic slides were processed using the Avizo 9.5.0 software.
4
Etoricoxib Immediate Release Tablets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ETO (MW = 358.842 g/mol; logP = 2.79 and pKa: 4.96) [14 ] (ETO) active pharmaceutical ingredient (API) and the test and reference pharmaceutical products were kindly provided by a pharmaceutical company. All drug products were immediate release (IR) tablets containing 120 mg of ETO with conventional excipients in customary amounts. The reference product is commercialized in Europe as Arcoxia® and it contains calcium hydrogen phosphate (anhydrous) as excipient, which was not included in the candidate generic test products, which contained only microcrystalline cellulose as diluent. All other tablet core excipients (microcrystalline cellulose, magnesium stearate, and sodium croscarmellose) where qualitatively the same, but quantitatively different. Acetonitrile and methanol were purchased from Sigma (Barcelona, Spain). Sodium hydroxide (NaOH), sodium chloride (NaCl), and sodium dihydrogen phosphate monohydrate (NaH2PO4·H2O) were received from Sigma-Aldrich (St. Louis, MO, USA). Purified water (i.e., filtrated and deionized) was used in the analysis methods and in dissolution studies to prepare the dissolution media (Millipore, Billerica, MA, USA).
5
Characterization of Fluorescent Cell-Labeling Beads
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
White, spherical CA beads
(diameter: 2 mm; density: 1.3 g/cm3) were purchased from
Cospheric, USA. DCF sodium salt, 5-([4,6-dichlorotriazin-2-yl]amino)
fluorescein hydrochloride (DTAF), sodium bicarbonate, potassium hydroxide
(KOH) pellets, disodium phosphate heptahydrate (Na2HPO4·7 H2O), and sodium dihydrogen phosphate monohydrate
(NaH2PO4·H2O) were purchased
from Sigma-Aldrich, Austria, and used as received. Advanced Dulbecco’s
modified Eagle’s medium (DMEM) and fetal bovine serum (FBS)
were purchased from Thermo Fisher, Germany. Human-derived skin fibroblasts
(ATCC CCL-119, Detroit 551, LGC Standard) were purchased from ATCC,
UK. Ultrapure water (18.2 MΩ cm) from a Milli-Q water purification
system (Millipore Corporation, USA) was used for deacetylation, buffer
preparation, and drug release experiments.
(diameter: 2 mm; density: 1.3 g/cm3) were purchased from
Cospheric, USA. DCF sodium salt, 5-([4,6-dichlorotriazin-2-yl]amino)
fluorescein hydrochloride (DTAF), sodium bicarbonate, potassium hydroxide
(KOH) pellets, disodium phosphate heptahydrate (Na2HPO4·7 H2O), and sodium dihydrogen phosphate monohydrate
(NaH2PO4·H2O) were purchased
from Sigma-Aldrich, Austria, and used as received. Advanced Dulbecco’s
modified Eagle’s medium (DMEM) and fetal bovine serum (FBS)
were purchased from Thermo Fisher, Germany. Human-derived skin fibroblasts
(ATCC CCL-119, Detroit 551, LGC Standard) were purchased from ATCC,
UK. Ultrapure water (18.2 MΩ cm) from a Milli-Q water purification
system (Millipore Corporation, USA) was used for deacetylation, buffer
preparation, and drug release experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!