Anti cd40l mab clone mr1

Manufactured by BioXCell

Anti-CD40L mAb (clone MR1) is a monoclonal antibody that binds to the CD40 ligand (CD40L) protein. CD40L is a member of the tumor necrosis factor (TNF) superfamily and is expressed on the surface of activated T cells, platelets, and other cell types. The anti-CD40L mAb (clone MR1) can be used in research applications to study the role of the CD40-CD40L interaction in various biological processes.

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Anti cd40l mab, by BioXCell (1 mentions)

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Anti-CD40L (11 citations) Anti-CD40L (MR1) (7 citations) CD40L (4 citations) Anti-CD40L mAb (3 citations)

4 protocols using anti cd40l mab clone mr1

Tolerance Induction in TEa TCR Mice

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TEa TCR transgenic mice do not express the homologous antigen and thus do not normally have nTreg or iTreg. TEa mice were tolerized with an intravenous (i.v.) injection of 1×10⁷ BALB/c spleen cells and 0.25 mg anti-CD40L mAb (clone MR1; Bio X Cell, West Lebanon, NH) on day 0, and another 0.25 mg anti-CD40L mAb injection on day 3. On day 7, mice were euthanized and spleen and LN harvested and analyzed for iTreg generation by flow cytometry.

Xiong Y., Ahmad S., Iwami D., Brinkman C.C, & Bromberg J.S. (2016). T-bet regulates natural Treg afferent lymphatic migration and suppressive function. Journal of immunology (Baltimore, Md. : 1950), 196(6), 2526-2540.

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Heterotopic Heart Transplantation Protocol

Cited 3 times

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Heterotopic Heart transplantation was performed as previously described (4 (link), 19 (link), 30 (link), 31 (link)). In some experiments recipients were treated with either 250μg anti-CD40L mAb (clone MR1, BioXcell) i.v. on the day prior surgery or 250μg CTLA4-Ig (abatacept) on day +1. Rejection was defined as the day on which a palpable heartbeat was no longer detectable and was confirmed by histological examination.

Llaudo I., Fribourg M., Medof M.E., Conde P., Ochando J, & Heeger P.S. (2018). C5aR1 regulates migration of suppressive myeloid cells required for costimulatory blockade-induced murine allograft survival. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 19(3), 633-645.

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Cardiac Transplant Tolerance Induction

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BALB/c hearts were transplanted as fully vascularized heterotopic grafts into C57BL/6 mice as previously described (Corry et al., 1973 (link)). Recipient mice were treated with 250 μg anti-CD40L mAb (clone MR1, BioXcell) for tolerance induction on days 0, 2, and 4 as previously described (Jiang et al., 2011 (link)). Graft function was monitored every other day by abdominal palpation. Untreated control mice received hamster IgG in PBS. Rejection was defined as complete cessation of a palpable beat and confirmed by direct visualization at laparotomy.

Conde P., Rodriguez M., van der Touw W., Jimenez A., Burns M., Miller J., Brahmachary M., Chen H.M., Boros P., Rausell-Palamos F., Yun T.J., Riquelme P., Rastrojo A., Aguado B., Stein-Streilein J., Tanaka M., Zhou L., Zhang J., Lowary T.L., Ginhoux F., Park C.G., Cheong C., Brody J., Turley S.J., Lira S.A., Bronte V., Gordon S., Heeger P.S., Merad M., Hutchinson J., Chen S.H, & Ochando J. (2015). DC-SIGN+ Macrophages Control the Induction of Transplantation Tolerance. Immunity, 42(6), 1143-1158.

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Tolerance Induction in Heart Transplantation

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BALB/c hearts were transplanted as fully vascularized heterotopic grafts into C57BL/6 mice as previously described (37 (link)). Recipient mice were treated with 250μg anti-CD40L mAb (clone MR1, BioXcell, West Lebanon, NH) for tolerance induction on days 0, 2, and 4 as previously described (38 (link)). Graft function was monitored every other day by abdominal palpation. Untreated control mice received hamster IgG in PBS. Rejection was defined as complete cessation of a palpable beat and confirmed by direct visualization at laparotomy.

Braza M.S., Conde P., Garcia M., Cortegano I., Brahmachary M., Pothula V., Fay F., Boros P., Werner S.A., Ginhoux F., Mulder W.J, & Ochando J. (2018). Neutrophil derived CSF1 induces macrophage polarization and promotes transplantation tolerance. American Journal of Transplantation, 18(5), 1247-1255.

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