A LECO Pegasus 4D GC-ToFMS instrument equipped with an Agilent 6890 N GC and a high speed ToF mass spectrometer (LECO, St. Joseph, MI, USA) was used for data acquisition. The chromatographic column was a SLB-IL60 (30 m × 0.25 mm ID × 0.2 μm) capillary column (Supelco, Bellefonte, PA, USA). A GC inlet was equipped with a 0.75 mm ID narrow-bore deactivated Sky® liner (Restek, Bellefonte, PA, USA). Desorption of SPME fibers was carried out at 270 °C for 15 min. Helium was used as a carrier gas with a flow rate of 1.5 mL/min. Oven temperature was set to 35 °C for 5 min, to 100 °C at 15 °C/min (5 min hold), to 260 °C at 15 °C/min (6 min hold). Transfer line and ion source temperatures were set to 200 and 260 °C, respectively. The acquisition range was set to 35–550 u, electron ionization was enabled, and the acquisition rate was 20 spectra/sec. Data acquisition and processing were performed with ChromaTOF (version 4.5) software.
Tof mass spectrometer
Manufactured by Leco
Sourced in United States
The ToF mass spectrometer is an analytical instrument used for the detection and identification of molecules based on their mass-to-charge ratio. It functions by ionizing sample compounds and separating the ions based on their time-of-flight, which is determined by their mass-to-charge ratio. The ToF mass spectrometer provides high-resolution mass analysis for a wide range of applications.
Automatically generated - may contain errors
2 protocols using tof mass spectrometer
1
GC-ToFMS Analysis of Volatile Compounds
2
Metabolite Profiling of Soybean Organs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Metabolites were extracted from five different organs (1st [L1], 2nd [L2], and 3rd [L3] trifoliate leaves; stems [S]; and roots [R]) of soybean plants at the V3 growth stage (25 mg/sample) using extraction medium (3:1:1 [v/v/v] methanol/chloroform/water). Extraction and derivatization were performed as described (Kusano et al., 2007 , 2011 ). Metabolites were detected using a GC instrument (Model 6890, Agilent Technologies, Palo Alto, CA, USA) fitted with an Rxi‐5Sil MS column (0.25‐mm i.d., 0.25‐ µm film; Restek) coupled to a TOF mass spectrometer (Leco, St. Joseph, MI, USA). Ten stable isotope reference compounds were used as internal standards (Kusano et al., 2007 ). All raw data in netCDF format were pre‐processed by hyphenated data analysis (Jonsson et al., 2005 , 2006 ). The obtained data matrix was normalized and summarized using the cross‐contribution‐compensating multiple standard normalization method (Redestig et al., 2009 ). For metabolite identification, we cross‐referenced the obtained mass spectra with GC‐EI‐MS mass spectral and RI libraries (Schauer et al., 2005 ) in the Golm Metabolome Database (Kopka et al., 2005 ) and our own in‐house libraries. The reproducibility of GC‐TOF‐MS analysis was assessed with three biological replicates per experiment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!