Be0075 clone rb6 8c5

The virulence of the various C. albicans strains and the effects of gefitinib and SGX523 were determined using our standard mouse model of oropharyngeal candidiasis(Solis and Filler, 2012 (link); Swidergall et al., 2021 (link); Zhu et al., 2012 (link)). All studies were performed using male Balb/c mice that were randomly assigned to the different experimental groups. For studies with immunocompetent or phagocyte-depleted mice, the animals were inoculated with calcium alginate swabs that had been soaked in HBSS containing 2×10⁷ organisms/ml and for experiments with mice that had been immunosuppressed with cortisone acetate, the animals were inoculated with calcium alginate swabs that had been soaked in HBSS containing 1×10⁶ organisms/ml. These mice were administered gefitinib and/or SGX523 by adding it to powdered mouse chow at final concentrations of 200 ppm and 120 ppm, respectively, starting at day −1 relative to infection. To deplete the mice of phagocytes, they were administered 80 μg of an anti-GR-1 antibody (#BE0075; clone RB6–8C5, Bio X Cell) intraperitoneally on day −1 relative to infection. Control mice were injected with a similar dose of an isotype control antibody (#BE0090, Clone LTF-2, Bio X Cell). The mice were sacrificed after 1, 2 or 5 days of infection, depending on the experiment, after which the tongues were excised, weighed, and quantitatively cultured.

The virulence of the various C. albicans strains and the effects of gefitinib and SGX523 were determined using our standard mouse model of oropharyngeal candidiasis [17 (link),22 (link),54 (link)]. All studies were performed using male Balb/c mice that were randomly assigned to the different experimental groups. For studies with immunocompetent or phagocyte-depleted mice, the animals were inoculated with calcium alginate swabs that had been soaked in HBSS containing 2x10⁷ organisms/ml and for experiments with mice that had been immunosuppressed with cortisone acetate, the animals were inoculated with calcium alginate swabs that had been soaked in HBSS containing 1x10⁶ organisms/ml. These mice were administered gefitinib and/or SGX523 by adding it to powdered mouse chow at final concentrations of 200 ppm and 120 ppm, respectively, starting at day -1 relative to infection. To deplete the mice of phagocytes, they were administered 80 μg of an anti-GR-1 antibody (#BE0075; clone RB6-8C5, Bio X Cell) intraperitoneally on day -1 relative to infection. Control mice were injected with a similar dose of an isotype control antibody (#BE0090, Clone LTF-2, Bio X Cell). The mice were sacrificed after 1, 2 or 5 days of infection, depending on the experiment, after which the tongues were excised, weighed, and quantitatively cultured.