To enable quantification of the dye concentration indicative of the antibody concentration in all spots of the array, the intensity must be determined in each spot. For this purpose, microarray slides were printed with a distinct pattern of 18 × 18 dots (150 μm diameter, 280 μm spacing). The slides were imaged using both the TinyArray imager and the commercial ArrayCam system (Grace Bio-Labs, Bend, OR). As the size and distances of the spots were known, the background-subtracted median spot fluorescence intensity was measured and quantified using Mapix (Innopsys) as previously described.25–27 (link) Mean fluorescence intensity (MFI) of the four replicate spots for each antigen was utilized for antigen heat map generation and data analysis.
Arraycam system
Manufactured by Grace Bio-Labs
The ArrayCam system is a laboratory instrument designed for imaging and data acquisition. It is capable of capturing high-quality images of biological samples, such as protein or DNA microarrays, with precision and accuracy.
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Lab products found in correlation
2 protocols using arraycam system
1
Quantitative Microarray Image Analysis
2
Quantifying Antibody Concentrations in Microarray
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To enable quantification of the dye concentration indicative of the antibody concentration in all spots of the array, the intensity must be determined in each spot. For this purpose, microarray slides were printed with a distinct pattern of 18 × 18 dots (150 μm diameter, 280 μm spacing). The slides were imaged using both the TinyArray imager and the commercial ArrayCam system (Grace Bio-Labs, Bend, OR). As the size and distances of the spots were known, the background-subtracted median spot fluorescence intensity was measured and quantified using Mapix (Innopsys) as previously described22 (link)–24 (link). Mean fluorescence intensity (MFI) of the four replicate spots for each antigen was utilized for antigen heat map generation and data analysis.
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