The extraction procedure for the KRG followed the international standard production process (ISO 19610). The six-year-old P. ginseng root extract (body 75% and root 25%) was prepared by repeated stemming and drying process from the Korea Ginseng Corporation (Daejeon, Republic of Korea). The extract was freeze-dried, and finally, we obtained a dark-brown powder (KRG). Ammonium bicarbonate, dithiothreitol (DTT), formic acid (FA), trifluoroacetic acid, ammonium formate, and urea were purchased from Sigma-Aldrich (St Louis, MO, USA). The HPLC-grade acetonitrile (ACN) and water were purchased from JT Baker (Phillipsburg, NJ, USA). Lyophilized trypsin and lys-C were obtained from Promega (Madison, WI, USA) and Waco (Osaka, Japan). Antibodies against HLA-DQB1/B2, MPO, vimentin, LCN2, BANF1, and GAPDH were purchased from Abcam (Cambridge, MA, USA) as well as HLA-DQB1 from Mybiosource (San Diego CA, USA). Antibodies directed against CRAMP (CAMP), RT1-B, and actin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Banf1
Manufactured by Abcam
Sourced in United States, United Kingdom
BANF1 is a protein that is involved in the transport of proteins between the nucleus and the cytoplasm. It plays a critical role in the regulation of cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Hplc grade acetonitrile acn, by Avantor (1 mentions)
Ammonium formate, by Merck Group (1 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Vimentin, by Abcam (1 mentions)
Gapdh, by Abcam (1 mentions)
Lys c, by Promega (1 mentions)
Lyophilized trypsin, by Promega (1 mentions)
Ecl kit, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin fraction 5, by HiMedia (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using banf1
1
Extraction and Analysis of Korean Red Ginseng
2
Western Blot Analysis of BANF1 and HBx
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The cells were harvested after 48 h of transfection using the mammalian protein extraction reagent (mPER; Thermo Fisher Scientific) supplemented with protease inhibitor cocktail (PIC, Thermo Fisher Scientific). Bicinchoninic acid (BCA) assay was performed for the estimation of protein concentration. Total cellular protein (40 μg per well) was run in the SDS-PAGE gels, transferred onto a PVDF membrane, blocked using bovine serum albumin fraction-V (HiMedia) and were incubated overnight with BANF1 (Abcam, Cambridge, UK), HBx (Santa Cruz Biotechnology, Dallas, TX, USA), or βactin (Santa Cruz). PVDF membranes were washed 6 × 5 min each and incubated with HRP-conjugated corresponding secondary antibodies and developed using ECL kit (Thermo Fisher Scientific) on photosensitive films.
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