Prior to MALDI TOF MS analysis, samples were spotted on an
MTP 384 ground steel Bruker
MALDI plate using the two-layer/overlay method: 0.4
μL of sample was deposited and allowed to dry, followed by deposition of 0.4
μL of matrix solution. Matrix solutions included 10 mg/mL of DHB and 10 mg/mL of THAP, with and without 10 mM LiCl
(aq). All matrix solutions were made in
n-propanol/MeOH (1:1, v/v). MALDI TOF MS analysis was done using an
ultrafleXtreme mass spectrometer (Bruker Daltonics, Bremen, Germany) in positive reflectron mode. Laser properties included a medium smartbeam at a frequency of 1 kHz for 6000 shots per sample. The laser power used was 62–64% for THAP and 50%–52% for DHB. Random walk, partial sample mode was enabled. The
m/z range was set to 440–1600. Sphingomyelin and ceramide abundance were calculated using a one-point calibration curve based on the addition of the internal standard mixture (EquiSPLASH lipidomix, SM(d36:2-d9) and Cer(d33:1-d7)), respectively. The lithium adduct ([M + Li]
+) was used for the calculation of abundance for both the analyte and internal standard. Data was analyzed via Bruker’s flexAnalysis Version 3.4 (Build 50). Univariate analysis was done using
Prism 6 (GraphPad, La Jolla, CA).
Tran A., Wan L., Xu Z., Haro J.M., Li B, & Jones J.W. (2020). Lithium Hydroxide Hydrolysis Combined with MALDI TOF Mass Spectrometry for Rapid Sphingolipid Detection. Journal of the American Society for Mass Spectrometry, 32(1), 289-300.