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Fei talos f200x microscope

Manufactured by Thermo Fisher Scientific
Sourced in United States

The FEI Talos F200X is an advanced transmission electron microscope (TEM) designed for high-resolution imaging and analysis of materials at the nanoscale. It features a high-brightness electron source, advanced optics, and a variety of detectors to provide detailed structural and chemical information about samples.

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4 protocols using fei talos f200x microscope

1

Synthesis and Characterization of Zn-Doped Cu2O Nanocrystals

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The Zn-decorated Cu2O NCs were prepared by a wet-chemical ligand-free method (see ESI) and were spray-coated on a carbon-based GDE (Sigracet 28BC). The Cu : Zn ratio was later quantified by comparing the absorption edge steps in X-ray absorption data at the Cu K-edge and the Zn K-edge, acquired in transmission mode. For the three bimetallic samples that were investigated, the obtained Zn percentages were 4%, 7% and 15%. The ex situ characterization of the NCs was done using a scanning transmission electron microscope (STEM, FEI Talos F200X microscope, Thermo Fisher Scientific) and an energy dispersive spectroscopy (EDX, SuperX 4 SDD EDX detector) featuring an XFEG field emission gun (200 kV) to determine the morphology and elemental distribution of the samples. The detectors in use were bright-field (BF), dark field (DF) and high angle annular dark-field (HAADF) detectors.
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2

Characterization of Cr2S3 Nanoflakes

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The morphologies of Cr2S3 nanoflakes were examined using Atomic Force Microscopy (AFM, Park system XE7, Suwon, South Korea). Transmission electron microscope (TEM) characterization was carried out on a FEI Talos f200x microscope (Thermo Fisher, San Francisco, CA, USA) operated at 200 KV. The X-ray photoelectron spectroscopy (XPS) spectra were recorded employing Al Kα radiation from a XSAM800 spectrometer (Kratos, Manchester, UK). The binding energy of samples was calibrated by C 1s peak. XRD patterns at variable temperatures were examined using Rigaku SmartLab (Tokyo, Japan). The magnetic properties were studied by a physical properties measurement system (PPMS, Quantum Design, San Diego, CA, USA) with a vibrating sample magnetometer option (Ever Cool II).
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3

Comprehensive Characterization of Recombinant Proteins

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PCR was performed in DNA Engine PCR amplifier and DNA was determined by Mini-Sub cell GT system and a Gel imaging system that were all obtained from Bio-Rad (Hercules, CA, USA). Electroporation was performed in Gene Pulser Xcell electroporator (Bio-Rad, Hercules, CA, USA). OD value was measured in Multiskan MK3 microplate reader (Thermo-Fisher, Waltham, MA, USA). Fluorescence intensity was measured in SpectaMax i3x micro-titer plate reader (Molecular Devices, Sunnyvale, USA). Characterization of rCDs was performed in Nicolet IS10 FT-IR Spectrometer (Thermo-Fisher, Waltham, MA, USA), FEI Talos F200X microscope (Thermo-Fisher, Hillsboro, OR, USA), ESCALAB 250XI electron spectrometer (Thermo Scientific, Waltham, MA, USA), and FLS1000/FSS FL spectrometer (Edinburgh Instruments Ltd, Livingston, UK).
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4

TiO2 Nanoparticle Characterization by TEM

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Powder TiO2 samples were dispersed in distilled water/ethanol and the suspension was treated in ultrasound for 5 min. A drop of very dilute suspension was placed on a holey-carbon-coated copper grid and dried by evaporation at ambient temperature.
Transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), and scanning transmission electron microscopy (STEM) were performed on a FEI Talos F200X microscope (Thermo Fisher Scientific, Waltham, MA, USA) operating at 200 keV. Images were recorded on a CCD camera with resolution of 4096 × 4224 pixels using the ‘User interface software package.’ An energy dispersive X-ray spectroscopy (EDX) system attached to the TEM operating in the STEM mode was used to analyze the chemical composition of the samples. High-angle annular dark-field (HAADF) image presented in the paper was captured in nanoprobe-TEM mode with a camera length of ~200 mm. All of the presented digital images were analyzed, but not processed.
The absorption coefficient of the light (α) of the newly synthesized nanocomposite was measured by ultraviolet-visible (UV-Vis) spectrophotometer Evolution 600 (Thermo Fisher Scientific, Waltham, MA, USA), in the electromagnetic spectrum range between 240 nm and 840 nm with the step of 1 nm and speed of 10 nm/min. Demineralized water was used as a reference during the measurements.
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