Antibodies and their conditions of use are as follows: mouse anti-His (western blot, 1 : 1000; 34660; Qiagen), rabbit anti-Brl1 (western blot, 1 : 1000; made in-house), rabbit anti-Tub2 (western blot, 1 : 1000; made in-house) and rabbit anti-GFP (western blot, 1 : 1000; Proteintech), rabbit anti-HA (western blot, 1 : 500; Proteintech), rabbit anti-GFP (immuno-EM, 1 : 5; gift from M. Seedorf, Zentrum für Molekulare Biologie, Heidelberg, Germany).
Rabbit anti ha
Manufactured by Proteintech
Sourced in Germany
Rabbit anti-HA is a primary antibody that specifically binds to the hemagglutinin (HA) tag. The HA tag is a commonly used epitope tag that can be fused to proteins to facilitate their detection and purification. Rabbit anti-HA is a useful tool for researchers working with HA-tagged proteins.
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Lab products found in correlation
Rabbit anti gfp, by Proteintech (1 mentions)
Polyvinylidene fluoride (pvdf), by Merck Group (1 mentions)
Radioimmunoprecipitation assay (ripa), by Beyotime (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Beyotime (1 mentions)
Rabbit anti sqstm1 p62, by Cell Signaling Technology (1 mentions)
Hrp conjugated anti rabbit igg, by Beyotime (1 mentions)
Rabbit anti lc3, by Cell Signaling Technology (1 mentions)
2 protocols using rabbit anti ha
1
Antibody Validation for Western Blot
2
Western Blot Analysis of Autophagy Proteins
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The procedure was performed as described previously and modification slightly (Zhang et al., 2017 (link)). Briefly, the cells were lysed with RIPA (Beyotime Biotechnology) containing protease inhibitor and 1% Phenylmethylsulfonylfluoride (PMSF , Beyotime Biotechnology), and the supernatant was separated by 12% SDS-PAGE electrophoresis. After electrophoresis, the target protein was transferred from PAGE gel to a nitrocellulose membrane (PVDF, Millipore, MO). Subsequently, the membrane was blocked and incubated with rabbit anti-HA (Proteintech Group, Rosemont, IL), rabbit anti-FLAG (Beyotime Biotechnology), rabbit anti-LC3 (Cell Signaling Technology, Danvers, MA), rabbit anti-SQSTM1/p62 (Cell Signaling Technology, Danvers, MA) or rabbit anti-β-actin antibodies (Beyotime Biotechnology), followed by incubation with HRP-conjugated anti-rabbit IgG (Beyotime Biotechnology). Finally, the protein signal was displayed by an enhanced chemiluminescence (ECL ) Western blotting analysis system.
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