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Di sodium phosphate

Manufactured by HiMedia
Sourced in India

Di-sodium phosphate is a chemical compound used as a laboratory reagent. It is a white, crystalline solid that is soluble in water. Di-sodium phosphate is commonly used as a buffering agent to maintain a specific pH in various laboratory procedures and experiments.

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3 protocols using di sodium phosphate

1

Biochemical Synthesis Procedures

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Salicylic acid, hydrogen peroxide, catechol, guaiacol, polyvinylpyrrolidone, tris buffer, sodium phosphate, and di-sodium phosphate were purchased from Himedia, Bangalore, India. All chemicals used were of analytical grade (≥99% purity).
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2

Metabolic Profile of Blood Cancer Cells

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Di-sodium phosphate, potassium dihydrogen phosphate, sodium chloride, magnesium sulfate heptahydrate, calcium chloride dihydrate, ammonium sulfate,  glucose and Nessler's reagent were obtained from HiMedia (Mumbai, India). Bradford reagent, L-asparagine and L-glutamine were obtained from Sigma Aldrich (St. Louis, USA). Blood cancer cell line K562 and normal cell line IEC-6 were procured from National Center for Cell Science (NCCS Pune, India).
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3

Preparation of Phosphate Buffer Solution

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Tz, Silicone oil, disodium phosphate, monosodium phosphate, and Glycine were obtained from Himedia chemicals, Bangalore, India and were used as received. The preparation of aqueous solution was done with double distilled deionized water. The stock solution of Tz (25 × 10−4 M), Glycine (25 × 10−3 M) were in the double distilled water. Phosphate buffer solution was prepared by mixing the suitable amount of 0.2 M monosodium phosphate and 0.2 M disodium phosphate.
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