Pdsred monomer membrane

Manufactured by Takara Bio

PDsRed-Monomer-Membrane is a fluorescent protein-based labeling reagent for visualizing cell membranes. It is derived from the Discosoma red fluorescent protein and designed to be a monomeric form that can be efficiently incorporated into cell membranes.

Automatically generated - may contain errors

Lab products found in correlation

Pcdna3.1 vector, by Thermo Fisher Scientific (1 mentions) Ha ubiquitin, by Addgene (1 mentions) Pcdna3.1 myc, by Thermo Fisher Scientific (1 mentions) Pcdna3.1 flag vector, by Thermo Fisher Scientific (1 mentions) Pdsred er, by Takara Bio (1 mentions) Quikchange site directed mutagenesis kit, by Agilent Technologies (1 mentions)

2 protocols using pdsred monomer membrane

Generation of rEag1 G469R mutant

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rEag1 cDNA (Dr. Olaf Pongs, Saarland University) was subcloned into the pcDNA3.1 vector (Invitrogen). The G469R mutation was generated using the QuikChange site-directed mutagenesis kit (Stratagene), followed by DNA sequence verification. Other cDNA constructs include pcDNA3-Myc human cullin 3/4 A/4B/7 (Addgene 19893, 19951, 19922, 20695), pDsRed-Monomer-Membrane (Clontech), and pCR3.1-Myc human Fbw8 (Dr. Zhen-Qiang Pan, Mount Sinai School of Medicine).

Hsu P.H., Ma Y.T., Fang Y.C., Huang J.J., Gan Y.L., Chang P.T., Jow G.M., Tang C.Y, & Jeng C.J. (2017). Cullin 7 mediates proteasomal and lysosomal degradations of rat Eag1 potassium channels. Scientific Reports, 7, 40825.

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Cloning and Mutagenesis of Eag1 and MKRN1

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cDNAs for rat Eag1 (Dr Olaf Pongs, Saarland University, Germany) and MKRN1 long isoform were subcloned into pcDNA3.1, pcDNA3.1-Myc, or pcDNA3.1-Flag vectors (Invitrogen). Disease-causing (G348R, G469R, and I467V) and glycosylation-deficient Eag1 mutations (N388Q and N406Q; Eag1-QQ), as well as MKRN1 short form and the catalytically inactive E3 ligase mutant MKRN1-H307E, were generated using the QuikChange site-directed mutagenesis kit (Agilent Technologies), followed by verification with DNA sequencing. The other cDNA constructs include pDsRed-Monomer-Membrane (Clontech), pDsRed-ER (Clontech Laboratories), and HA-Ubiquitin (Addgene).

Fang Y.C., Fu S.J., Hsu P.H., Chang P.T., Huang J.J., Chiu Y.C., Liao Y.F., Jow G.M., Tang C.Y, & Jeng C.J. (2021). Identification of MKRN1 as a second E3 ligase for Eag1 potassium channels reveals regulation via differential degradation. The Journal of Biological Chemistry, 296, 100484.

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