Propyl methyl cellulose

Male Sprague-Dawley rats (10 weeks old, n=15) were divided into three groups; Control group without prostatic inflammation (n=5), Placebo group with prostatic inflammation and placebo treatment (n=5) and Dutasteride group with prostatic inflammation and Dutasteride treatment (n=5). Rats were housed in plastic cages with soft bedding and free access to food and water under a 12/12 hour reversed light-dark cycle. Control rats were injected with 50 μl normal saline into each of bilateral ventral lobes of the prostate as sham operation. Prostatic inflammation was induced by 5% formalin solution injection at a volume of 50μl into each of bilateral ventral lobes of the prostate. Dutasteride group rats were treated with Dutasteride (Sigma-Aldrich Co.), which was dissolved in propyl methyl cellulose (Wako Pure Chemical Industries, Ltd.) at a dose of 0.5mg/kg daily from 2 days before intraprostatic formalin injection whereas Placebo rats received hydroxy propyl methyl cellulose (HPMC) only as vehicle treatment. After cystometry, bladder and ventral lobes of the prostate were harvested for evaluation of mRNA expression and histological analysis.