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Fluorinert fc 77

Manufactured by Merck Group

Fluorinert® FC-77 is a perfluorinated liquid produced by Merck Group. It is a clear, colorless, and odorless fluid with a high chemical and thermal stability. Fluorinert® FC-77 has a low surface tension and a high dielectric strength, making it suitable for use in various industrial applications.

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2 protocols using fluorinert fc 77

1

MRI Imaging of Heated Samples

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The MRI protocol for the present study has been previously described9 (link). Briefly, samples were inserted into a susceptibility-matched polyetherimide (ULTEM) sample holder containing Fluorinert® FC-77 (Sigma-Aldrich, St. Louis, MO) and heated to 37.0 ± 0.1 °C prior to data acquisition. The ULTEM holder also contained a Dulbecco’s phosphate-buffered saline (DPBS; Invitrogen) standard for normalizing weighted images. Imaging was performed using a 3T Philips Achieva system equipped with an 8-channel SENSE knee coil with sample temperature maintained at 37.0 ± 0.1 °C for the duration of the acquisition.
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2

Osteochondral Plug Imaging Protocol

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The 40 imaging-dedicated osteochondral plugs were imaged in a susceptibility-matched polyetherimide (ULTEM) sample holder containing four vertical sample wells 9 mm in diameter and 8.5cm deep. Three wells contained samples submerged in Fluorinert® FC-77 (Sigma–Aldrich, St. Louis, MO). The fourth well was filled with Dulbecco’s phosphate-buffered saline (DPBS; Invitrogen), a signal intensity (SI) standard. During handling and transport, all samples were maintained at 4°C to minimize sample degradation prior to imaging.
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