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100s electron microscope

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The JEOL 100S electron microscope is a high-performance instrument designed for advanced materials analysis. It utilizes an electron beam to magnify and image samples at the nanoscale level, providing detailed information about their structure and composition. The JEOL 100S is capable of producing clear, high-resolution images and data, making it a valuable tool for researchers and scientists in various fields.

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Lab products found in correlation

Orca hr camera, by Hamamatsu Photonics (2 mentions) Epon araldite, by Electron Microscopy Sciences (2 mentions) Ultracut microtome, by Leica (1 mentions) Ultracut e ultramicrotome, by JEOL (1 mentions)

5 protocols using 100s electron microscope

1

Immunogold Labeling of Cellular Proteins

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A cell monolayer was sequentially dehydrated in ascending alcohols and infiltrated for 4 h at RT with three changes of LR white acrylic resin hard (Sigma). Coverslips were covered with a cylindrical capsule filled with fresh LR white resin and polymerized at 60 °C for 24 h. Thin sections were collected on 400-mesh nickel grids, incubated for 30 min at RT in TBS blocking buffer (20 mM TRIS–HCl, pH 7.6, 225 mM NaCl and 1% bovine serum albumin) containing 5% normal goat serum and then incubated overnight at 4 °C with a polyclonal antibody recognizing primary monoclonal antibody (AC-1, 8 ng/ml). After a 10-min washing step in TBS blocking buffer, samples were incubated for 1 h at RT with EM goat anti-mouse IgG 10 nm gold. Control grids were also included in which the primary antibody was omitted. Sections were stained with uranyl acetate and examined by means of a JEOL 100S electron microscope (JEOL, Peabody, Mass., USA) operating at 80 kV.
Santoro A., Nicolin V., Florenzano F., Rosati A., Capunzo M, & Nori S.L. (2017). BAG3 is involved in neuronal differentiation and migration. Cell and Tissue Research, 368(2), 249-258.
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2

Ultrastructural Analysis of Myelinated Axons

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Randomly selected P90 mice from each group were euthanized with isoflurane, intracardially perfused with 2% glutaraldehyde and 2% paraformaldehyde in 0.1 M sodium phosphate buffer, pH 7.4. Brains were removed, fixed for 72 hours at 4°C. Coronal slices (~ 1 mm thickness) were cut and small pieces (~ 1×1 mm) containing CC and EC areas were dissected-out, post-fixed in 2 % OsO4 in PB for 2 hours at 4°C, dehydrated with alcohol and propylene oxide, and embedded in Epon-Araldite (Electron Microscopy Sciences, Hatfield, PA). Semi-thin sections were stained with toluidine blue; areas of interest were identified under the upright microscope for the blocks trimming. Ultrathin sections were stained with uranyl acetate and lead citrate, and examined using JEOL 100S electron microscope (JEOL, Peabody, MA) equipped with a Hamamatsu ORCA HR camera. The axonal myelination was evaluated by the calculation of the g-ratio: ratio of the inner axon diameter to the entire diameter of the axon with myelin sheath, as we described previously [7 (link)]. Axons with diameters less than 300 nm were not analyzed. At least, six images per animal captured at 20k magnification were used for quantification. A minimum of 50 axons per animal were analyzed.
Sosunov S.A., Niatsetskaya Z.V., Stepanova A.A., Galkin A.S., Juliano C.E., Ratner V.I, & Ten V.S. (2021). Developmental window of vulnerability to white matter injury driven by sublethal intermittent hypoxemia. Pediatric research, 91(6), 1383-1390.
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3

Imaging PS1 Localization by Immunoelectron Microscopy

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Adult CD1 mice were perfused with 4 % paraformaldehyde (PFA) before the cortices were excised and 1 mm-thick slices were fixed in a mixture of 0.1 % tetroxide, dehydrated, and embedded in araldite/dodecynyl succinic anhydride (DDSA) resin (Electron Microscopy Sciences, Hatfield, PA). Then, 70 nm-thick sections were cut on an ultracut microtome (Leica) and dried onto formar/carbon-coated mesh electron microscopy (EM) grids prior to immunostaining with anti-PS1 C-terminal fragment primary antibody (Sigma, P7854), followed by 6 nm-Ag-particles labeled secondary antibody (Aurion). Grids were negatively stained with 1 % filtered uranyl acetate in 70 % ethanol. The samples were examined and the images acquired using a JEOL 100S electron microscope (JEOL, USA, Peabody MA).
Kuzuya A., Zoltowska K.M., Post K.L., Arimon M., Li X., Svirsky S., Maesako M., Muzikansky A., Gautam V., Kovacs D., Hyman B.T, & Berezovska O. (2016). Identification of the novel activity-driven interaction between synaptotagmin 1 and presenilin 1 links calcium, synapse, and amyloid beta. BMC Biology, 14, 25.
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4

Ultrastructural Analysis of Myelinated Axons

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Randomly selected P90 mice from each group were euthanized with isoflurane, intracardially perfused with 2% glutaraldehyde and 2% paraformaldehyde in 0.1 M sodium phosphate buffer, pH 7.4. Brains were removed, fixed for 72 hours at 4°C. Coronal slices (~ 1 mm thickness) were cut and small pieces (~ 1×1 mm) containing CC and EC areas were dissected-out, post-fixed in 2 % OsO4 in PB for 2 hours at 4°C, dehydrated with alcohol and propylene oxide, and embedded in Epon-Araldite (Electron Microscopy Sciences, Hatfield, PA). Semi-thin sections were stained with toluidine blue; areas of interest were identified under the upright microscope for the blocks trimming. Ultrathin sections were stained with uranyl acetate and lead citrate, and examined using JEOL 100S electron microscope (JEOL, Peabody, MA) equipped with a Hamamatsu ORCA HR camera. The axonal myelination was evaluated by the calculation of the g-ratio: ratio of the inner axon diameter to the entire diameter of the axon with myelin sheath, as we described previously [7 (link)]. Axons with diameters less than 300 nm were not analyzed. At least, six images per animal captured at 20k magnification were used for quantification. A minimum of 50 axons per animal were analyzed.
Sosunov S.A., Niatsetskaya Z.V., Stepanova A.A., Galkin A.S., Juliano C.E., Ratner V.I, & Ten V.S. (2021). Developmental window of vulnerability to white matter injury driven by sublethal intermittent hypoxemia. Pediatric research, 91(6), 1383-1390.
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5

TEM Imaging of NP Uptake in MCF-7 Cells

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The cellular TEM images were obtained using a JEOL 100S electron microscope, and the samples were prepared according to the previous report.[11 (link)] Briefly, MCF-7 cells were seeded and incubated on 15-mm-diameter Theramanox coverslips (Nalge Nunc International), and placed in 24-well plates (100,000 cells per well) with 1 ml of serum containing media for 24 h before the experiment. The media was replaced by NPs in serum containing media and incubated for 3 h. The media was exchanged with new media, and the MCF7 cells were washed three times with PBS buffer. The cells were then fixed in 2 % glutaraldehyde with 3.75% sucrose in 0.1 M sodium phosphate buffer (pH 7.0) for 30 min and washed with 0.1 M PBS containing 3.75% sucrose, three times over 30 min. The fixed cells were postfixed in 1% osmium tetroxide with 5% sucrose in 0.05 M sodium phosphate buffer solution (pH 7.0) for 1 hour and then washed with DI water three times. They were dehydrated in a graded series of acetone (10% steps) and embedded in epoxy resin. The resin was polymerized at 70 °C for 12 h. Ultrathin sections (70 nm) were obtained with a Reichert Ultracut E Ultramicrotome then imaged under a JEOL 100S electron microscope.
Kim C.S., Le N.D., Xing Y., Yan B., Tonga G.Y., Kim C., Vachet R.W, & Rotello V.M. (2014). The role of surface functionality in nanoparticle exocytosis. Advanced healthcare materials, 3(8), 1200-1202.
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