Dp70 colour ccd camera

Time-lapse recordings of the cell cultures were performed on a computer-controlled Leica DM IRB inverted microscope equipped with a Marzhauser SCAN-IM powered stage and a 10x N-PLAN objective with 0.25 numerical aperture and 17.6 mm working distance. The microscope was coupled to an Olympus DP70 colour CCD camera. Cell cultures were kept in a stage-top mini incubator at 37°C in humidified 5% CO₂ atmosphere. Phase contrast images were collected every 10 minutes from each microscopic field for durations up to 3-4 days.

Figure 1 shows the inverted bioluminescence microscope used in our studies (Luminoview LV200; Olympus, Tokyo, Japan). Bioluminescence emitted from live cells in a culture dish was collected by an objective lens and the light passed through an imaging lens that then transmitted the image to a CCD camera. The objective lenses used in this study were UPlanFLN 40×/NA 1.30 Oil and UPlanFLN 100×/NA 1.30 Oil (Olympus). The DP70 colour CCD camera (Olympus) and ImagEM EM-CCD camera (C9100–13; Hamamatsu Photonics, Shizuoka, Japan) were equipped for an LV200 microscope. The imaging lens was developed with a focal length of 36 mm with an NA of 0.2. A stage-top incubator with temperature and CO₂ gas controllers (MI-IBC-IF; Tokai Hit Co., Shizuoka, Japan) was added to the sample stage. The observation area was covered with a dark box.