Genemapper software

Manufactured by Promega

GeneMapper software is a data analysis tool designed to analyze DNA fragment analysis data generated by genetic analyzers. It provides a user-friendly interface for processing, visualizing, and interpreting the data.

Automatically generated - may contain errors

Lab products found in correlation

Powerplex 16 system, by Promega (2 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Mem nonessential amino acids solution 100x, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

2 protocols using genemapper software

Cell Culture Maintenance Protocol

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All cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) or RPMI-1640 supplemented with 10% FBS, 20 mmol/L L-glutamine, 10 U/mL penicillin, and 10 μg/mL streptomycin and maintained at 37°C under 5% CO₂. Cell line identities are regularly confirmed by short tandem repeat profiling using the PowerPlex16 system and GeneMapper software (Promega).

Eleveld T.F., Oldridge D.A., Bernard V., Koster J., Daage L.C., Diskin S.J., Schild L., Bentahar N.B., Bellini A., Chicard M., Lapouble E., Combaret V., Legoix-Né P., Michon J., Pugh T.J., Hart L.S., Rader J., Attiyeh E.F., Wei J.S., Zhang S., Naranjo A., Gastier-Foster J.M., Hogarty M.D., Asgharzadeh S., Smith M.A., Guidry Auvil J.M., Watkins T.B., Zwijnenburg D.A., Ebus M.E., van Sluis P., Hakkert A., van Wezel E., van der Schoot C.E., Westerhout E.M., Schulte J.H., Tytgat G.A., Dolman M.E., Janoueix-Lerosey I., Gerhard D.S., Caron H.N., Delattre O., Khan J., Versteeg R., Schleiermacher G., Molenaar J.J, & Maris J.M. (2015). Relapsed neuroblastomas show frequent RAS-MAPK pathway mutations. Nature genetics, 47(8), 864-871.

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Cell Culture Protocol for Mycoplasma-Free Lines

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Cell lines (derived from the American Type Culture Collection (ATCC)) were cultured in DMEM (Life Technologies, cat. 41965039) supplemented with 10% FBS (Life Technologies, cat. 10270106), 20 mM L-glutamine (Life Technologies, cat. 25030024), 10 U/ml penicillin and 10 μg/ml streptomycin (Life Technologies, cat. 15140122) and MEM Non-essential amino acids solution (100x, Life Technologies, Cat. No. 11140035), and maintained at 37°C under 5% CO₂. Cell line identities were regularly confirmed by short tandem repeat (STR) profiling using the PowerPlex16 system and GeneMapper software (Promega) and cell lines were regularly screened for mycoplasma.

Eleveld T.F., Vernooij L., Schild L., Koopmans B., Alles L.K., Ebus M.E., Dandis R., van Tinteren H., Caron H.N., Koster J., van Noesel M.M., Tytgat G.A., Eising S., Versteeg R., Dolman M.E, & Molenaar J.J. (2023). MEK inhibition causes BIM stabilization and increased sensitivity to BCL-2 family member inhibitors in RAS-MAPK-mutated neuroblastoma. Frontiers in Oncology, 13, 1130034.

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