In order to monitor the release of components during storage of cellular suspensions, different measurements were performed in untreated and PEF treated samples.
Turbidity of the suspension during the storage was measured by the absorbance at 600-nm (Abs600) to monitor leakage of cellular content. Absorbance at 260-nm (Abs260) and 280-nm (Abs280) of the supernatant was measured in order to monitor the presence of intracellular material outside the cell (Aronsson et al., 2005 (link)).
The concentration of mannoproteins in the extracellular medium was determined after hydrolyzing the supernatant with sulfuric acid (final concentration 1.5 M) at 100°C for 90 min. Cooled samples were neutralized with NaOH 3 M. Quantitative analysis of mannose was conducted by an enzymatic method (D -Mannose, D -Fructose, and D -Glucose assay procedure, Megazyme International, Wicklow, Ireland) (Dupin et al., 2000 (link)).
Turbidity of the suspension during the storage was measured by the absorbance at 600-nm (Abs600) to monitor leakage of cellular content. Absorbance at 260-nm (Abs260) and 280-nm (Abs280) of the supernatant was measured in order to monitor the presence of intracellular material outside the cell (Aronsson et al., 2005 (link)).
The concentration of mannoproteins in the extracellular medium was determined after hydrolyzing the supernatant with sulfuric acid (final concentration 1.5 M) at 100°C for 90 min. Cooled samples were neutralized with NaOH 3 M. Quantitative analysis of mannose was conducted by an enzymatic method (