Kanamycin sulfate

Unless stated otherwise, At-SPHERE isolates were
cultivated at room temperature on R-2A agar (Sigma-Aldrich) or at 28°C in
R-2A broth (0.5 g yeast extract (Oxoid), 0.5 g Proteose peptone No. 3 (Becton,
Dickinson and Company), 0.5 g Casamino acids (Becton, Dickinson and Company),
0.5 g D-glucose monohydrate (Sigma-Aldrich), 0.5 g starch (from potato, Fluka),
0.3 g sodium pyruvate (Sigma-Aldrich), 0.3 g K₂HPO₄(AppliChem) and 0.05 g magnesium sulfate heptahydrate (Sigma-Aldrich) dissolved
in 1 L deionized water), both supplemented after sterilization with 0.5% (v/v)
methanol (R-2A+M). Minimal medium was prepared as described previously^{66 (link)} and supplemented with 5 mM
maltose (Fluka). For Leaf374 cultivation, minimal medium agar was supplemented
with vitamins (500 μg L^-1 D-pantothenic acid hemi calcium
salt, 100 μg L^-1 biotin, 400 μg L^-1riboflavin, 400 μg L^-1 thiamine HCl, 200 μg
L^-1 pyridoxal HCl 150 μg L^-1 p-amino benzoic
acid, 200 μg L^-1 cobalamin, 50 μg L^-1 lipoic
acid, 150 μg L^-1 nicotinic acid and 100 μg
L^-1 folic acid). For selective growth of Leaf272, R-2A+M was
supplemented with 50 μg mL^-1 rifampicin (Sigma-Aldrich), since
this strain has a natural resistance towards this antibiotic.
E. coli BL21 (DE3) gold was cultivated in LB-Lennox at
37°C supplemented with 50 μg mL^-1 kanamycin sulfate
(AppliChem).