Originpro 9.0g software

Steady-state activation curves were analyzed by fitting the Boltzmann equation to each individual recording using the OriginPro 9.0G software (Northampton, United States): $\frac{I}{I_{max}}=\frac{\frac{I}{I_{max,satV}}}{1+e^{\frac{z\delta F\left(V-V_{1/2}\right)}{RT}}}$
I/I_max is the relative current, I/I_{max, satV} is the relative current at a saturating voltage and the actual cAMP concentration, V_1/2 is the voltage of half-maximum activation, and zδ the effective gating charge. F, R, and T are the Faraday constant, the molar gas constant, and the temperature in Kelvin, respectively. The time courses of current activation were fitted with a single exponential starting after an initial delay using the OriginPro 9.0G software (Northampton, United States): $I\left(t\right)=A{e}^{\frac{-t}{\tau }}$
A is the amplitude, t the time, and τ the time constant for activation.
Experimental data are given as mean ± standard error of the mean (SEM). Statistical analysis was performed by an unpaired Student’s t-test. A value of p < 0.05 was accepted as statistically significant.