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Fam labeled primer probes

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FAM-labeled primer/probes are oligonucleotide sequences that are labeled with the fluorescent dye FAM (6-carboxyfluorescein). These labeled sequences are designed to bind to specific target DNA or RNA molecules, allowing for their detection and quantification in various molecular biology applications, such as real-time PCR and other fluorescence-based assays.

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Lab products found in correlation

Smartcycler, by Cepheid (2 mentions) Rneasy kit, by Qiagen (2 mentions)

2 protocols using fam labeled primer probes

1

Quantitative Real-Time RT-PCR Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA) and was reverse transcribed with a Superscript kit (Invitrogen/Life Technologies), both according to the manufacturer’s instructions. Real-time RT-PCR was performed using Taqman gene expression assays with FAM-labeled primer/probes (Applied Biosystems, Foster City, CA) detected in a SmartCycler (Cepheid, Sunnyvale, CA) and normalized to the 18S rRNA signal. The fold change in the mRNA expression was determined using the ΔΔCt method.12 (link)
Das S., Sarkar A., Choudhury S.S., Owen K.A., Derr-Castillo V.L., Fox S., Eckmann L., Elliott M.R., Casanova J.E, & Ernst P.B. (2015). Engulfment and Cell Motility Protein 1 (ELMO1) Has an Essential Role in the Internalization of Salmonella Typhimurium Into Enteric Macrophages That Impact Disease Outcome. Cellular and Molecular Gastroenterology and Hepatology, 1(3), 311-324.
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2

Quantitative Real-Time RT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA) and reverse transcribed with a Superscript kit (Invitrogen), both according to the manufacturer’s instructions. Real-time RT PCR was performed using Taqman gene expression assays with FAM labeled primer/probes (Applied Biosystems) detected in a SmartCycler (Cepheid) and normalized to the 18S rRNA signal. The fold change in mRNA expression was determined using the ΔΔCt method12 (link).
Das S., Sarkar A., Choudhury S.S., Owen K.A., Derr-Castillo V.L., Fox S., Eckmann L., Elliott M.R., Casanova J.E, & Ernst P.B. (2015). Engulfment and Cell Motility Protein 1 (ELMO1) Has an Essential Role in the Internalization of Salmonella Typhimurium Into Enteric Macrophages That Impact Disease Outcome. Cellular and Molecular Gastroenterology and Hepatology, 1(3), 311-324.
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