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2 protocols using 5 5 dithiobis 2 nitrobenzoic acid dtnb dihydrogen phosphate

1

Antioxidant Assay Protocol

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Succinicacid, potassium dihydrogen and monohydrogen phosphate, glycine, pyrogallol, hydrogen peroxide, trichloroacetic acid (TCA) and ethylenediaminetetra-acetic acid (EDTA), sulphosalicylic acid, and thiobarbituric acid (TBA) were purchased from BDH, England. 5,5-dithiobis-2-nitrobenzoic acid (DTNB), dihydrogen phosphate, trichloroacetic acid, carbon tetrachloride, and thiobarbituric acid were purchased from Merck Company, Darmstadt. Melatonin was obtained from MP Biomedicals, LLC., France. Folic acid was purchased from Sigma Chemical Company, USA. The rest of all other chemicals used were of analytical grade.
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2

Antioxidant Potential of Thymoquinone

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Succinic acid, potassium dihydrogen and mono-hydrogen phosphate, glycine, pyrogallol, hydrogen peroxide, trichloroacetic acid (TCA) and ethylenediaminetetraacetic acid (EDTA) sulphosalicylic acid and thiobarbituric acid (TBA) were purchased from BDH, England. 5,5-dithiobis-2-nitrobenzoic acid (DTNB), dihydrogen phosphate, trichloroacetic acid, carbon tetrachloride, and thiobarbituric acid were obtained from Merck Company, Darmstadt (Germany). Rest all other chemicals including carbon tetrachloride (CCl4) were from Sigma-Aldrich Company (St. Louis. MO, USA). Thymoquinone (TQ) was naturally derived from the Nigella sativa leaves extract in the present study.
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