Anti taz

For western blotting, the perineurium and epineurium were removed from sciatic nerves prior to snap-freezing and storage at −80 °C. Sciatic nerves and rat SCs were lysed in RIPA buffer, containing protease and phosphatase inhibitors. Western blot analysis was performed as described previously52 (link). Gapdh (Millipore MAB374, dilution 1:1,000) was used as an input control. The antibodies used were anti-TAZ (Novus biologicals, NB11058359, dilution 1:1,000), YAP (Rabbit; Cell Signaling Technology, #4912, dilution 1:1,000), anti-p-YAP (Cell Signaling Technology, #13008S, dilution 1:1,000) anti-EGR2/KROX20 (Rabbit; Santa Cruz, sc-20690, dilution 1:1,000) and Anti-Gαs (Rabbit; Santa Cruz sc-823, dilution 1:1,000). Secondary antibodies conjugated to HRP were from Jackson ImmunoResearch Laboratories (dilution of secondary antibodies is 1:10,000). Full-size images for the main figures and Supplementary Figs are presented in Supplementary Fig. 9.

Anti-YAP/TAZ (D24E4) dual antibodies were used at 1:1000 (Cell Signaling Technologies, catalog# 8418S), anti-YAP (D8H1X) antibody used at 1:1000 (Cell Signaling Technologies catalog# 14074T), anti-phosphorylated YAP S127 at 1:1000 (Cell Signaling Technologies, catalog# 4911), anti-TAZ (Novus, catalog# NB110-58359), anti-phosphorylated TAZ S89 (E1X9C) at 1:1000 (Cell Signaling Technologies, catalog# 59971), anti-BZLF1 antibody was used at 1:500 (Santa Cruz, catalog# sc-53904), anti-BRLF1 antibody at 1:2000 (isolated from rabbits injected with peptide sequence EDPDEETSSQAVKALREMAD), anti-BMRF1 antibody at 1:2000 (Millipore-Sigma, catalog# MAB8186), anti-LATS1 antibody at 1:1000 (Cell Signaling Technologies, catalog# 9153), anti-phosphorylated LATS S909 at 1:1000 (Cell Signaling Technologies catalog# 9157), PAN-TEAD antibodies at 1:1000 (Cell Signaling Technologies catalog# 13295S), anti-tubulin antibody at 1:4000 (Sigma, catalog# T5168), anti-β actin antibody at 1:5000 (Sigma catalog# 5441), and anti-HSP90 (F8) at 1:1000 (Santa Cruz catalog# sc-13119). The secondary antibodies used were Horseradish peroxide (HRP)- labeled goat anti-mouse antibody at 1:5000 (Thermo Scientific catalog# 31430), HRP- labeled donkey anti-goat antibody (Santa Cruz catalog# sc-2056, 1:5000), and HRP- labeled anti-rabbit antibody (Fisher Scientific catalog# 31460 1:10000).