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Dulbecco s modified eagle s culture medium

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Dulbecco's modified Eagle's culture medium is a widely used cell culture medium formulation. It is designed to support the growth and maintenance of a variety of cell types in vitro. The medium provides essential nutrients, growth factors, and other components necessary for cell proliferation and survival.

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Lab products found in correlation

Fetal bovine serum (fbs), by Capricorn (1 mentions) Penicillin streptomycin, by Capricorn (1 mentions)

Spelling variants of this product

Dulbecco’s modified (44 citations) Dulbecco′s Modified Eagle′s culture medium (DMEM), (3 citations) Dulbecco's Modified Eagle Medium (DMEM) cell culture medium (2 citations) Dulbecco’s Modified Eagle Medium (DMEM)culture medium (2 citations) Dulbecco's Modified Eagle's cell culture medium (2 citations)

4 protocols using dulbecco s modified eagle s culture medium

1

Preparation of Graphene Oxide Hydrocolloids

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GO colloid (GO) (purity 99.99%) was purchased from Nanopoz (Poznan, Poland) and rGO was purchased from Graphene Laboratories Inc. (Ronkonkoma, NY, USA) and dispersed in ultrapure water to prepare a 1.0 mg/mL solution.
After 30 min of sonification, the hydrocolloids of GO and rGO were diluted to different concentrations with 1× Dulbecco’s modified Eagle’s culture medium (Sigma-Aldrich, St Louis, MO, USA) immediately before exposure to cells.
Kutwin M., Sosnowska M.E., Strojny-Cieślak B., Jaworski S., Trzaskowski M., Wierzbicki M., Chwalibog A, & Sawosz E. (2021). MicroRNA Delivery by Graphene-Based Complexes into Glioblastoma Cells. Molecules, 26(19), 5804.
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2

Culturing SW620 Colorectal Cancer Cells

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Human SW620 colorectal adenocarcinoma cells derived from lymph node metastasis were obtained from American Type Culture Collection and maintained in Dulbecco's Modified Eagle's culture medium (Sigma-Aldrich, Dorset, UK) supplemented with 2 mM L-glutamine, 100 U ml−1 penicillin, 0.2 mg ml−1 streptomycin and 10% (v/v) fetal bovine serum in a humidified atmosphere with 5% CO2 at 37 °C.
Papaevangelou E., Almeida G.S., Jamin Y., Robinson S.P, & deSouza N.M. (2015). Diffusion-weighted MRI for imaging cell death after cytotoxic or apoptosis-inducing therapy. British Journal of Cancer, 112(9), 1471-1479.
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3

Synthesis and Characterization of Platinum Nanoparticles

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The colloid of platinum nanoparticles was purchased from Nano-koloid (Warsaw, Poland). This material is produced by a patented electric non-explosive method (Polish patent 380649) from high-purity metal (99.9999%) and high-purity demineralized Milli-Q water. Graphene oxide powder (GO) (purity 99.99%) was purchased from CEZEMAT (Warsaw, Poland) and dispersed in ultrapure water to prepare a 1.0 mg/mL solution.
After 30 minutes of sonification, the hydrocolloids of GO-NP-Pt, NP-Pt, and GO were diluted to different concentrations with 1× Dulbecco’s modified Eagle’s culture medium (Sigma-Aldrich, St. Louis, MO, USA) immediately prior to exposure to cells.
Kutwin M., Sawosz E., Jaworski S., Wierzbicki M., Strojny B., Grodzik M., Ewa Sosnowska M., Trzaskowski M, & Chwalibog A. (2019). Nanocomplexes of Graphene Oxide and Platinum Nanoparticles against Colorectal Cancer Colo205, HT-29, HTC-116, SW480, Liver Cancer HepG2, Human Breast Cancer MCF-7, and Adenocarcinoma LNCaP and Human Cervical Hela B Cell Lines. Materials, 12(6), 909.
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4

Preparation of CaP_Si Powder Suspensions

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Following the sterilization under ultraviolet light (15 min), CaP_Si powders were soaked in high-glucose Dulbecco’s modified Eagle’s culture medium (DMEM, Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS, Capricorn Scientific, Ebsdorfergrund, Germany) and 1% penicillin–streptomycin (Capricorn Scientific), at a concentration of 10 mg/mL. After incubation at 4 °C for 24 h, CaP_Si powders suspensions were centrifuged at 300× g for 5 min in order to spin down the powders.
Ressler A., Bauer L., Prebeg T., Ledinski M., Hussainova I., Urlić I., Ivanković M, & Ivanković H. (2022). PCL/Si-Doped Multi-Phase Calcium Phosphate Scaffolds Derived from Cuttlefish Bone. Materials, 15(9), 3348.
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