Prosensor hd

After extraction, the teeth were immediately fixed in 4% neutral buffered formalin (VWR) for a minimum of 24 h and prepared for macroscopic photography and histological processing according to the previously published protocol (Demant et al., 2021 (link)). Briefly, a radiograph was first obtained of each specimen in a buccal‐lingual direction using a Planmeca Prosensor. HD, Planmeca ProX intraoral X‐ray unit and the Planmeca Romexis software (Plandent). The exposure time was 0.6 s, with 6KV and 8 mA as parameters. Each tooth was then sectioned in half through the central part of the occlusal cavitated lesion in a mesio‐distal direction, using a high‐precision saw (MIKRO‐TRENN). Each sectioned half was photographed, and the specimens were subsequently decalcified in 10% EDTA (pH 6.9) and processed for histology. Each macroscopically sectioned half was sectioned serially into 4 μm sections, starting from the central part of the carious lesion until complete exhaustion of the specimens. The number of obtained sections varied greatly from specimen to specimen due to differences in tooth type and section plane. The sectioning procedure was carried out using a sliding microtome (Leica Biosystems). A detailed description of the tissue processing methods has been described elsewhere (Demant et al., 2021 (link)).