Radioimmunoprecipitation buffer (Beyotime) was used to extract total protein from GC tissues and cell lines. Protein concentrations were quantified with a BCA Protein Assay Kit (Thermo Fisher Scientific). The primary antibodies used in this study included an antibody against C‐E‐Cad (1:1000; This study), E‐Cad (1:1000; Abcam), N‐Cad (1:1000; AbcamK), Snail (1:1000; Abcam), Slug (1:1000; Abcam), Vimentin (1:1000; Abcam), p‐AKTS473 (1:1000; Abcam), p‐AKTT308 (1:1000; Abcam), p‐PDK1 (1:1000; Abcam), AKT (1:1000; Abcam), PDK1 (1:1000; Abcam), p‐Smad2/3 (1:1000; Abcam), Smad2/3 (1:1000; Abcam). β‐actin (1:5000; Sigma‐Aldrich) was used as a control.
P pdk1
Manufactured by Abcam
P-PDK1 is a lab equipment product. It is a recombinant protein that represents the catalytic domain of the enzyme PDK1 (Phosphoinositide-Dependent Kinase 1). PDK1 is a serine/threonine protein kinase that plays a key role in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
P smad2 3, by Abcam (1 mentions)
Smad2 3, by Abcam (1 mentions)
E cad, by Abcam (1 mentions)
P akt t308, by Abcam (1 mentions)
Snail, by Abcam (1 mentions)
Pakt s473, by Abcam (1 mentions)
β actin, by Merck Group (1 mentions)
Vimentin, by Abcam (1 mentions)
Radioimmunoprecipitation buffer, by Beyotime (1 mentions)
Bcl xl, by Abcam (1 mentions)
Chemiluminescence kit, by Beyotime (1 mentions)
C myc, by Abcam (1 mentions)
Cyclin d1, by Abcam (1 mentions)
2 protocols using p pdk1
1
Protein Extraction and Western Blot Analysis
2
Protein Expression Analysis in ARPE-19 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total proteins were obtained from ARPE-19 cells and retinal samples from mice. We used the NE-PER Nuclear and Cytoplasmic Extraction Reagents (Beyotime Biotechnology) to prepare the nuclear and cytoplasmic extract from the ARPE-19 cells or retinal samples. A bicinchoninic acid assay kit was used to measure the protein concentration. We used SDS-PAGE to separate equal amounts of protein. Specific antibodies against YAP, EGFR, p-EGFR, Mst1, Lats1, CTGF, Cry61, p-PDK1, Cyclin D1, C-Myc, Bcl-xl, and RhoA were obtained from Abcam Biotechnology. The target proteins were measured using a chemiluminescence kit (Beyotime Biotechnology). The blots were captured with a Leica DMI4000B instrument (Olympus Soft Imaging Solutions GmbH).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!