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3 protocols using anti golgin97 d8p2k

1

Immunofluorescence Microscopy Antibodies

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Rabbit anti-Ror2 61 (link) and anti-IFT20 31 (link) antibodies were prepared as described previously. Sheep anti-TGN46 and rabbit anti-Giantin antibodies have been described previously62 (link). Following antibodies were purchased commercially: mouse anti-GM130 (35, BD), anti-Cortactin (4F11, Millipore), anti-γ-tubulin (GTU-88, Sigma), anti-tyrosinated tubulin (TUB01A2, Sigma), anti-AKAP450 (15, BD), anti-GFP (JL-8, Clontech), anti-acetylated tubulin (6-11B-1, Sigma), anti-Myc (9E10, Santa Cruz), anti-Golgin-97 (CDF4, Thermo), and Alexa Fluor 647-conjugated anti-GM130 (5G8, MBL); rabbit anti-IFT20 (13615-1-AP, Proteintech), anti-Arl13B (ab83879, Abcam), anti-GM130 (PM061, MBL), anti-γ-tubulin (T5192, Sigma), anti-Golgin-97 (D8P2K, Cell Signaling Technology), and HRP-conjugated anti-α-tubulin (PM054-7, MBL).
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2

Antibodies for Microtubule-associated Proteins

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To detect MTCL1 and MTCL2, anti-KIAA0802 (sc-84865) from Santa Cruz Biotechnology and anti-SOGA1 (HPA043992) from Sigma-Aldrich were used, respectively. To detect other proteins, the following antibodies were used: anti-α-tubulin (sc-32293), anti-acetylated tubulin (sc-23950), anti-MAP4 (sc-67152), anti-GFP (sc-9996) and anti-CLASP2 (sc-376496) from Santa Cruz Biotechnology; anti-V5 (R960-25) from Thermo Fisher Scientific; anti-GM130 (610822) and anti-GS28 (611184) from preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted September 7, 2021. ; https://doi.org/10.1101/2020.08.19.251967 doi: bioRxiv preprint BD Transduction Laboratories; anti-GAPDH (5G4) from HyTest Ltd.; anti-giantin (ab37266) and anti-pericentrin (ab4448) from Abcam; anti-GCC185 from Bethyl Laboratories; anti-Flag (F3165) from Sigma-Aldrich; anti--tubulin (MAB3408) from Merk Millipore; anti-CLASP1 (MAB9736) from Abnova; anti-AKAP450 from Novus Biologicals; anti-Golgin97 (D8P2K) from Cell Signaling Technology.
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3

Detecting MTCL1 and MTCL2 Proteins

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To detect MTCL1 and MTCL2, anti-KIAA0802 (sc-84865) from Santa Cruz Biotechnology and anti-SOGA1 (HPA043992) from Sigma-Aldrich were used, respectively. To detect other proteins, the following antibodies were used: anti-α-tubulin (sc-32293), anti-acetylated tubulin (sc-23950), anti-MAP4 (sc-67152), anti-GFP (sc-9996) and anti-CLASP2 (sc-376496) from Santa Cruz Biotechnology; anti-V5 (R960-25) from Thermo Fisher Scientific; anti-GM130 (610822) and anti-GS28 (611184) from preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted September 7, 2021. ; https://doi.org/10.1101/2021.09.07.459224 doi: bioRxiv preprint BD Transduction Laboratories; anti-GAPDH (5G4) from HyTest Ltd.; anti-giantin (ab37266) and anti-pericentrin (ab4448) from Abcam; anti-GCC185 from Bethyl Laboratories; anti-Flag (F3165) from Sigma-Aldrich; anti--tubulin (MAB3408) from Merk Millipore; anti-CLASP1 (MAB9736) from Abnova; anti-AKAP450 from Novus Biologicals; anti-Golgin97 (D8P2K) from Cell Signaling Technology.
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