Spop-knockout MEF was a gift from Dr. Jun Yan (Model Animal Research Center of Nanjing University, China). Human cell lines were purchased from ATCC and maintained with 1% penicillin and streptomycin (Gibco Life Technologies, 15140122) at 37 °C with 5% CO2. A498 cells were cultured in MEM/EBSS medium (HyClone, SH30024.01) supplemented with 10% fetal bovine serum (FBS, Gibco, 10100154) and 2× nonessential amino acids (Gibco,11140050). HK2, MEF, and 786-O cells were cultured in DMEM/F12 medium (HyClone) supplemented with 10% FBS (Gibco). Caki-1, Caki-2, and SKOV3 cells were cultured in McCoy’s 5A medium (KeyGENE BioTECH). 293T cells were cultured in DMEM (Gibco,12100046) supplemented with 10% FBS (Wisent, 085-150), 1× glutamine (Gibco, 35050061), and 1 mM sodium pyruvate (Gibco, 11360070).
Sh30024
Manufactured by Thermo Fisher Scientific
The SH30024.01 is a laboratory equipment product from Thermo Fisher Scientific. It serves as a core function in laboratory settings, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mannitol, by Merck Group (1 mentions)
M4125, by Merck Group (1 mentions)
G6152, by Merck Group (1 mentions)
Cl 0090, by Procell (1 mentions)
Pm150410, by Thermo Fisher Scientific (1 mentions)
A3160802, by Thermo Fisher Scientific (1 mentions)
2 protocols using sh30024
1
Cell Culture Conditions for Multiple Cell Lines
2
Glucose and Osmotic Stress on Keratinocytes
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The human keratinocyte (HaCaT) cell line was purchased from Procell Life Science & Technology (CL-0090) and cultured with Minimum Essential Medium (MEM; Procell Life Science & Technology, PM150410) containing 10% fetal bovine serum (Gibco, A3160802). Normal human epithelial keratinocytes (NHEK) were purchased from Beijing Beina Chuanglian Biotechnology Institute (BNCC340593) and cultured with Minimum Essential Medium with Earle’s Balanced Salts (MEM/EBBS; Hyclone, SH30024.01) containing 10% fetal bovine serum (Gibco, A3160802). Cells were incubated in humidified condition of 37°C with 95% air and 5% CO2. For experiments, HaCaT cells or NHEK were treated with different concentrations of glucose (Sigma-Aldrich, G6152; normal glucose: 5.6 mM of glucose; mid-high glucose: 15 mM of glucose; high glucose: 30 mM glucose). mannitol (Sigma-Aldrich, M4125; 15 mM: 5.6 mM of glucose + 9.4 mM of mannitol; 30 mM: 5.6 mM of glucose + 24.4 mM of mannitol) was used as the osmotic control for mid-high glucose and high glucose.
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