Bio plex human cytokine plex panel

Manufactured by Bio-Rad

The Bio-Plex Human Cytokine Plex Panel is a multiplex assay system designed to quantify multiple cytokines simultaneously in a single sample. The panel allows for the detection and measurement of a variety of human cytokines using a small sample volume.

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Lab products found in correlation

Multiplex analyzer, by Bio-Rad (2 mentions) Enzyme immunoassay, by R&D Systems (1 mentions) Modular platform, by Roche (1 mentions) Chromogenic limulus amebocyte lysate assay, by Lonza (1 mentions)

2 protocols using bio plex human cytokine plex panel

Multiplex Cytokine Analysis in Plasma

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Plasma levels of tumor necrosis factor (TNFα) and interleukin (IL)-6, IL-8, and IL-12 were analyzed using a multiplex cytokine assay (Bio-Plex Human Cytokine Plex Panel; Bio-Rad Laboratories Inc., Hercules, CA). The samples were analyzed on a Multiplex Analyzer (Bio-Rad Laboratories) according to instructions from the manufacturer.

Macpherson M.E., Hov J.R., Ueland T., Dahl T.B., Kummen M., Otterdal K., Holm K., Berge R.K., Mollnes T.E., Trøseid M., Halvorsen B., Aukrust P., Fevang B, & Jørgensen S.F. (2020). Gut Microbiota-Dependent Trimethylamine N-Oxide Associates With Inflammation in Common Variable Immunodeficiency. Frontiers in Immunology, 11, 574500.

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Quantification of Soluble Immune Markers

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Plasma levels of soluble (s) CD14 and sCD25 were quantified in duplicates by enzyme immunoassays obtained from R&D Systems (Minneapolis, MN). Tumor necrosis factor (TNF) and interleukin (IL)-6, IL-8, and IL-12 were analyzed using a multiplex cytokine assay (Bio-Plex Human Cytokine Plex Panel; Bio-Rad Laboratories Inc., Hercules, CA), analyzed on a Multiplex Analyzer (Bio-Rad Laboratories) according to instructions from the manufacturer. High sensitivity C reactive protein (CRP) was analyzed on a MODULAR platform (Roche Diagnostics, Basel, Switzerland). LPS was analyzed by Limulus Amebocyte Lysate chromogenic assay (Lonza, Walkersville, MD) according to the manufacturer’s instructions, with the following modifications: samples were diluted tenfold to avoid interference with background colour and preheated to 68 °C for 10 min prior to analysis to dissolve immune complexes.

Macpherson M.E., Skarpengland T., Hov J.R., Ranheim T., Vestad B., Dahl T.B., Fraz M.S., Michelsen A.E., Holven K.B., Fevang B., Berge R.K., Aukrust P., Halvorsen B, & Jørgensen S.F. (2023). Increased Plasma Levels of Triglyceride-Enriched Lipoproteins Associate with Systemic Inflammation, Lipopolysaccharides, and Gut Dysbiosis in Common Variable Immunodeficiency. Journal of Clinical Immunology, 43(6), 1229-1240.

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