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Alanine aminotransferase alt

Manufactured by Horiba
Sourced in United States

Alanine aminotransferase (ALT) is a laboratory test used to measure the activity of the enzyme ALT in the blood. ALT is found primarily in the liver and is released into the bloodstream when the liver is damaged or inflamed.

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2 protocols using alanine aminotransferase alt

1

Carbon Tetrachloride-Induced Liver Injury

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Carbon tetrachloride (reagent grade 99.9%) and thioacetamide (ACS reagent >99.0%) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) assay kits were purchased from Pointe Scientific, Canton, MI, USA.
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2

Serum Biomarker Profiling Protocol

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Blood was allowed to clot, and supernatant was collected after centrifugation at 4 °C. Serum FGF21 levels were determined using a Rat/Mouse FGF-21 ELISA kit (R&D Systems, Minneapolis MN), according to the manufacturer's instructions. α-Feto protein/AFP was measured using a mouse Quantikine ELISA Kit (R&D Systems, Minneapolis MN). Alanine aminotransferase (ALT) (Pointe Scientific, Canton MI), aspartate aminotransferase (AST) (Pointe Scientific, Canton MI), triglycerides, total cholesterol (StanBio Laboratory, Boerne, TX) and nonesterified fatty acids (Wako Diagnostics, Richmond, VA) were measured in duplicate using enzyme colorimetric assays as per the directions provided by each manufacturer.
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