HPLC was performed using an Agilent 1260 infinity series stack equipped with an Agilent 1260 binary pump and degasser. The flow rate was set to 1.0 mL min−1 and samples were injected using Agilent 1260 autosampler with a 20 μL injection volume. The temperature of the column was set at 37 °C. The HPLC was fitted with a phenomenex Lunar C18 column (150 × 4.6 mm) with 5 m packing (100Ǻ). Detection was achieved using an Agilent 1260 variable wavelength detector. UV detection was monitored at λ = 309 nm. Methods were edited and run using Agilent OpenLAB online software and data was analysed using Agilent OpenLAB offline software. Mobile phase solvents used were HPLC grade (ACN was ‘far UV’) and consisted of mobile phase A: 100% ACN, 0.04% TFA; mobile phase B: 100% water, 0.04% TFA with a gradient of 5 to 95% ACN over 30 min. All data was elaborated using Prism9.
1260 binary pump and degasser
Manufactured by Agilent Technologies
The 1260 binary pump and degasser is a laboratory equipment from Agilent Technologies. It is designed to deliver precise and consistent flow of mobile phases for liquid chromatography applications. The binary pump provides reliable and accurate flow control, while the integrated degasser removes dissolved gases from the mobile phase.
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Lab products found in correlation
1260 infinity series stack, by Agilent Technologies (2 mentions)
1260 variable wavelength detector, by Agilent Technologies (2 mentions)
1260 autosampler, by Agilent Technologies (1 mentions)
Lunar c18 column, by Phenomenex (1 mentions)
Agilent 1260 autosampler, by Agilent Technologies (1 mentions)
C18 column, by Agilent Technologies (1 mentions)
2 protocols using 1260 binary pump and degasser
1
HPLC Analysis of Organic Compounds
2
HPLC Characterization of Organic Compounds
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HPLC was performed using an Agilent 1260 infinity series stack equipped with an Agilent 1260 binary pump and degasser. The flow rate was set to 1.0 mL min -1 and samples were injected using Agilent 1260 autosampler with a 100 μL injection volume. The temperature of the column was set at 37 °C.
The HPLC was fitted with an Agilent C18 column (100 x 4.6 mm) with 5 micron packing (100Ǻ).
Detection was achieved using an Agilent 1260 variable wavelength detector. UV detection was monitored at λ = 309 nm. Methods were edited and run using Agilent OpenLAB online software and data was analysed using Agilent OpenLAB offline software. Mobile phase solvents used were HPLC grade (ACN was 'far UV') and consisted of mobile phase A: 100 % ACN, 0.04 % TFA; mobile phase B: 100 % water, 0.04 % TFA with a gradient of 1 to 80 % ACN over 30 minutes.
The HPLC was fitted with an Agilent C18 column (100 x 4.6 mm) with 5 micron packing (100Ǻ).
Detection was achieved using an Agilent 1260 variable wavelength detector. UV detection was monitored at λ = 309 nm. Methods were edited and run using Agilent OpenLAB online software and data was analysed using Agilent OpenLAB offline software. Mobile phase solvents used were HPLC grade (ACN was 'far UV') and consisted of mobile phase A: 100 % ACN, 0.04 % TFA; mobile phase B: 100 % water, 0.04 % TFA with a gradient of 1 to 80 % ACN over 30 minutes.
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