All reagents were used
as procured from the vendor unless otherwise mentioned. PVC (high
molecular weight), hyperbranched PEI (MW: 25,000), NAC, trioctyl trimellitate
(TOTM), tetrahydrofuran (THF), 1-ethyl-3-(3-dimethyl aminopropyl)
carbodiimide (EDC), N-hydroxysuccinimide (NHS), and
Tryptic soy and Luria–Bertani (LB) broth and agar were purchased
from Sigma-Aldrich (St. Louis MO, USA). SNAP was purchased from Pharma
block (Hatfield, PA, USA). Human fibroblasts [BJ cells, American type
culture collection (ATCC) CRL-2522], Staphylococcus
aureus (ATCC 6538), and Escherichia
coli (ATCC 25922) were procured from ATCC (Manassas
VA, USA). Dulbecco’s modified Eagle’s medium and penicillin–streptomycin
were from Thermo Fisher Scientific (Waltham MA, USA). FBS was obtained
from VWR (Atlanta, GA USA). All aqueous solutions were prepared in
Milli-Q water (18.2 MΩ) and purified using a Mettler Toledo
(Columbus, OH USA) apparatus. The drip flow bioreactor apparatus was
acquired from BioSurface Technologies Corporation (Model: DFR-110-6,
Bozeman, MT, USA).
as procured from the vendor unless otherwise mentioned. PVC (high
molecular weight), hyperbranched PEI (MW: 25,000), NAC, trioctyl trimellitate
(TOTM), tetrahydrofuran (THF), 1-ethyl-3-(3-dimethyl aminopropyl)
carbodiimide (EDC), N-hydroxysuccinimide (NHS), and
Tryptic soy and Luria–Bertani (LB) broth and agar were purchased
from Sigma-Aldrich (St. Louis MO, USA). SNAP was purchased from Pharma
block (Hatfield, PA, USA). Human fibroblasts [BJ cells, American type
culture collection (ATCC) CRL-2522], Staphylococcus
aureus (ATCC 6538), and Escherichia
coli (ATCC 25922) were procured from ATCC (Manassas
VA, USA). Dulbecco’s modified Eagle’s medium and penicillin–streptomycin
were from Thermo Fisher Scientific (Waltham MA, USA). FBS was obtained
from VWR (Atlanta, GA USA). All aqueous solutions were prepared in
Milli-Q water (18.2 MΩ) and purified using a Mettler Toledo
(Columbus, OH USA) apparatus. The drip flow bioreactor apparatus was
acquired from BioSurface Technologies Corporation (Model: DFR-110-6,
Bozeman, MT, USA).