Total RNA was extracted from tissues or cells using Trizol reagent (Takara, Dalian, China). The expression of target gene mRNA was detected by the 7500 fast real-time polymerase chain reaction (PCR) system (Applied Biosystems, USA) using a one-step SYBR primescript RT-PCR Kit (RR064A, Takara, Dalian, China). β-actin was used as an endogenous control gene, and the primers used in the study are shown in Table S1 . The multiple changes were calculated using relative quantification (2-∆∆Ct).
7500 fast real time polymerase chain reaction
Manufactured by Thermo Fisher Scientific
Sourced in United States
The 7500 Fast Real-Time PCR System is a laboratory instrument used for performing real-time polymerase chain reaction (PCR) analysis. It is designed to rapidly amplify and detect specific DNA sequences. The system provides fast thermal cycling capabilities and can be used for a variety of real-time PCR applications.
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Lab products found in correlation
2 protocols using 7500 fast real time polymerase chain reaction
1
Real-time qPCR for Gene Expression
2
Quantification of Inflammatory Markers in ADSCs
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Total RNA was extracted from human ADSCs, NCM460 cells, and mouse colonic tissue using standard Trizol-based protocols, and complementary DNA was generated by reverse-transcriptase (RT) reaction (Promega, Madison, WI). An Applied Biosystems 7500 Fast Real-time Polymerase Chain Reaction (PCR) System was used with Fast Universal PCR Master Mix and validated TaqMan Gene Expression Assays for the detection of interleukin (IL) 1β, CCL2, CXCL1, IL6, IL17A, CCL23, lactoferrin, and vascular endothelial growth factor (VEGF) A (Applied Biosystems, Foster City, CA). Resulting data were calculated by using the 2-[delta][delta]Ct method, and the level of mRNA expression was normalized to Rn18S (Applied Biosystems). Expression of Rn18S was consistent across the treatments and conditions studied.
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