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Fluoview image stitching software

Manufactured by Olympus

Fluoview image stitching software is a tool designed to combine multiple microscope images into a single, seamless high-resolution image. The software automatically aligns and blends adjacent images, allowing users to capture and visualize larger field-of-views.

Automatically generated - may contain errors

2 protocols using fluoview image stitching software

1

Confocal Imaging and Quantification of Anterograde Tracing

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Images were taken with a confocal laser-scanning microscope (FV1200, Olympus) equipped with diode laser lines, and UPlanSApo (20×, NA 0.75) and PlanApoN (60×, NA 1.4, oil-immersion) objective lenses (Olympus). To avoid cross talk between multiple fluorophores, Alexa Fluor 488, Cy3, and Alexa Fluor 647 fluorescent signals were acquired sequentially using the 473, 559, and 647 nm excitation laser lines, respectively. All images show single optical sections. For quantification of anterograde tracing, we obtained images with a 20× objective and then created images of the entire hippocampus with a Fluoview image stitching software (Olympus). For analysis, the separate colour components were converted to greyscale, and the area of eYFP- and TH-positive elements were measured with Integrated Morphometry Analysis module (MetaMorph software, Molecular Devices).
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2

Confocal Imaging and Quantification of Anterograde Tracing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were taken with a confocal laser-scanning microscope (FV1200, Olympus) equipped with diode laser lines, and UPlanSApo (20×, NA 0.75) and PlanApoN (60×, NA 1.4, oil-immersion) objective lenses (Olympus). To avoid cross talk between multiple fluorophores, Alexa Fluor 488, Cy3, and Alexa Fluor 647 fluorescent signals were acquired sequentially using the 473, 559, and 647 nm excitation laser lines, respectively. All images show single optical sections. For quantification of anterograde tracing, we obtained images with a 20× objective and then created images of the entire hippocampus with a Fluoview image stitching software (Olympus). For analysis, the separate colour components were converted to greyscale, and the area of eYFP- and TH-positive elements were measured with Integrated Morphometry Analysis module (MetaMorph software, Molecular Devices).
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