The specimens (from subcutaneous implantation and calvarial bone-defect repair models) were fixed with 4% formalin, decalcified with 10% EDTA and subsequently embedded in paraffin. Coronal sections (6-μm thick) were stained with haematoxylin and eosin. For immunofluorescence analysis of OCN expression in newly formed tissue in the defect area, the slides were de-paraffinized and cooked in citrate buffer (2.1 M citric acid, pH 6.0) at 120 °C for 30 min for antigen retrieval. After blocking in serum, the sections were incubated overnight at 4 °C with a rabbit polyclonal primary antibody to OCN (1:100, Abcam). After three washes in PBS, the sections were incubated with FITC-conjugated donkey antibody to rabbit IgG (1:400, Santa Cruz) for 1 h. NC experiments were performed by omitting the primary antibody. The sections were mounted with the medium containing DAPI (Vector Laboratories) and then examined under a confocal microscope (Eclipse C1 Plus, Nikon).
Rabbit polyclonal primary antibody to ocn
Manufactured by Abcam
Rabbit polyclonal primary antibody to OCN. This antibody specifically recognizes the Osteocalcin (OCN) protein.
Automatically generated - may contain errors
2 protocols using rabbit polyclonal primary antibody to ocn
1
Histological Analysis of Bone Regeneration
2
Histological Analysis of Bone Regeneration
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The specimens (from subcutaneous implantation and calvarial bone-defect repair models) were fixed with 4% formalin, decalcified with 10% EDTA and subsequently embedded in paraffin. Coronal sections (6-μm thick) were stained with haematoxylin and eosin. For immunofluorescence analysis of OCN expression in newly formed tissue in the defect area, the slides were de-paraffinized and cooked in citrate buffer (2.1 M citric acid, pH 6.0) at 120 °C for 30 min for antigen retrieval. After blocking in serum, the sections were incubated overnight at 4 °C with a rabbit polyclonal primary antibody to OCN (1:100, Abcam). After three washes in PBS, the sections were incubated with FITC-conjugated donkey antibody to rabbit IgG (1:400, Santa Cruz) for 1 h. NC experiments were performed by omitting the primary antibody. The sections were mounted with the medium containing DAPI (Vector Laboratories) and then examined under a confocal microscope (Eclipse C1 Plus, Nikon).
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