Si erbb2

ERBB2 knockdown was generated in target cells through the transfection of si-ERBB2 synthesized by GenePharma (Shanghai, China). Ascramble sequence (si-NC) was used as anegative control. miR-488 overexpression or inhibition was generated in target cells by the transfection of miR-488 mimics or inhibitors synthesized by GenePharma. Regarding NFκB1 overexpression, the full-length human RELA cDNA was cloned in pcDNA3.1 expression vector using ahomologous recombination method. The empty vector pcDNA3.1 was used as anegative control. For cell transfection, 1 × 10⁵ cells were seeded in a6-well cell culture plate, 24 h later, cells were transfected with 50 pmol final concentration miR488-5p mimics, miR488-5p inhibitor or si-ERBB2 through 5 μl per well lipofectamine 3000 (Invitrogen). Following incubation with the RNA lipofectamine 3000 complex for 6 h, cells were further cultured with fresh complete medium for 48 h and harvested for further laboratory analysis. The sequence of siRNAs and primers for plasmid construction are listed in Table S1.