Phencyclidine

Nicotine free base was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Phencyclidine was purchased from Alomone Labs (Jerusalem, Israel). VETEC Química Fina Ltda (Rio de Janeiro, RJ, Brazil) was the source for all other reagents.

Ketamine (Ceva, Paulínia, SP, Brazil), xylazine (Syntec, Tamboré, SP, Brazil), flunixin (Ucb/Vet, Jaboticabal, SP, Brazil) and enrofloxacin (Venco, Londrina, PR, Brazil) are specifically for veterinary use. Nicotine-free base, dithiothreitol (DTT), iodoacetamide (IAA), formic acid, sodium deoxycholate and triethylammonium bicarbonate buffer (TEAB) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Phencyclidine was purchased from Alomone Labs (Jerusalem, Israel). Osmotic minipumps came from Alzet (Cupertino, CA, USA). Promega Biotecnologia do Brasil (São Paulo, SP, Brazil) was the source of trypsin, and Thermo Fischer Scientific (Waltham, MA, USA) was the source of the resin column model POROS 20 R2 and Qubit protein assay kit. The nanoEase Symmetry C18 TRAP column (5 µm, 180 µm × 20 mm), the nanoAcquity BEH130 C18 reversed-phase column (1.7 µm, 75 µm × 100 mm), [Glu1]-Fibrinopeptide B human (GFP) and Total Recovery vials were purchased from Waters Corp (Milford, MA, USA). Acetonitrile came from Merck HGaA (Darmstadt, Germany).