Anti gfap antibody

We analyzed the astrocytic cytoskeletal genotype and treatment-related changes within the hippocampal NVU of 3×Tg-AD and WT mice by measuring the surface area, intensity, and processes number of GFAP positive profiles. For the quantitative histochemical analysis, two coronal brain sections per mouse, cut at 25 µm intervals throughout the hippocampi (1.9–2.0 mm posterior to bregma), were used. The anti-GFAP antibody (Biolegend, #835301) with dilution 1:1000 served to determine the glial cytoskeletal profiles and treatment-related changes. Parallel confocal planes with step of 0.35 µm were scrutinized to characterize morphological features of the GFAP-positive objects. The NVU-associated astroglial end-feet were evaluated with ZEN 2.5. The end-feet thickness was measured (four points per one NVU), averaged, and tested statistically. Two representative (clearly seen in each plane) cells (each animal) from Cornus Ammonis 1 (CA1) hippocampal area were chosen for 3D reconstruction and analysis with Imaris 9.3 (Oxford Instruments plc, Abingdon, UK). The cell body volume, stain intensity, and number of astroglia processes were quantified and compared statistically.

Purified anti-Nestin Antibody, cat. 656802 (Biolegend, San Diego, CA, USA); Anti-GFAP Antibody, cat. 840001 (Biolegend, San Diego, CA, USA); Anti-TAGLN antibody, Sigma-Aldrich, cat. HPA019467 (Merck Life Science, Amsterdam, the Netherlands); Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal, Sigma-Aldrich, cat. A5228 (Merck Life Science, Amsterdam, the Netherlands); Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Texas Red, Invitrogen, cat. T-2767 (Thermo Fisher Scientific, Waltham, MA, USA); Alexa Fluor^® 488 Goat anti-mouse IgG (minimal x-reactivity) Antibody, cat. 405319 (Biolegend, San Diego, CA, USA).