The tumor cells in a single cell suspension were isolated from the each xenograft within 2 hours by using the gentleMACs Dissociator and Tumor Dissociation Kit (Miltenyi Biotec Inc., Auburn, CA) according to the manufacturer’s guidelines. 0.5 × 106 cells per sample for flow cytometry analysis were as follows: a) unstained; b) stained with mouse IgG1-PE/-FITC; c) stained with anti-human CD44-PE; d) stained with anti-human CD24-FITC; and e) stained with anti-human CD44-PE/CD24-FITC (Miltenyi Biotec Inc., Auburn, CA). The fluorescence intensity of these cell samples was analyzed by the Gallios flow cytometer (Beckman Coulter, Inc., Brea, CA). The ALDEFLUOR kit (Stemcell Technologies) was used for the identification of cancer stem cells from MDA-MB-231 xenografts by flow cytometry analysis.
Anti human cd24 fitc
Manufactured by Miltenyi Biotec
Sourced in Germany
Anti-human CD24-FITC is a fluorescently labeled antibody that binds to the CD24 surface antigen on human cells. It is used for the identification and analysis of CD24-expressing cell populations in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Gentlemacs dissociator and tumor dissociation kit, by Miltenyi Biotec (1 mentions)
Gallios flow cytometer, by Beckman Coulter (1 mentions)
Aldefluor kit, by STEMCELL (1 mentions)
Anti human cd44 fitc, by Miltenyi Biotec (1 mentions)
Human msc analysis kit, by BD (1 mentions)
Anti human cd133 apc, by Miltenyi Biotec (1 mentions)
Accuri c6, by BD (1 mentions)
2 protocols using anti human cd24 fitc
1
Isolation and Characterization of Tumor Cell Subpopulations
2
Identification of Cancer Stem Cell Markers
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Cultured cells were washed with phosphate-buffered saline (PBS) and resuspended in DMEM/F12 supplemented with 10% fetal bovine serum (FBS) at a density of 1×105 cells/100 µL. Single cells were stained using antibodies against CSCs; anti-human CD133-APC (Miltenyi Biotec, NRW, Germany), anti-human CD44-FITC (Miltenyi Biotec), and anti-human CD24-FITC (Miltenyi Biotec), and the Human MSC Analysis Kit (BD Biosciences, NJ, USA); CD90-FITC, CD105-PerCP-Cy5.5, and CD73-APC. Cells were incubated with the antibodies for 15 min at room temperature, washed with PBS, and analyzed using a BD Accuri C6 flow cytometer (BD Biosciences) and the software FlowJo v.10.
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