The HUVECs were purchased from American Type Culture Collection (ATCC; Manassas, USA) and cultured in the EGM02 medium (CC03162; Lonza, Cologne, Germany). miRNA-451 mimic and inhibitor (GenePharma, Shanghai, China) were transfected into the cells using Lipofectamine 2000 (11668027; Thermo Fisher Scientific, Waltham, USA). Then, the HUVECs were divided into 7 groups:
-group 1: cells cultured with medium supplemented with 10% FCS (Gibco, Rockville, USA); -group 2: cells cultured with medium supplemented with 10% rat serum from burn group; -group 3: cells cultured with medium supplemented with 10% rat serum from control group; -group 4: cells transfected with miRNA-451 mimic and cultured with medium supplemented with 10% rat serum from burn group; -group 5: cells transfected with scramble control for miRNA-451 mimic and cultured with medium supplemented with 10% rat serum from burn group; -group 6: cells transfected with miRNA-451 inhibitor and cultured with medium supplemented with 10% rat serum from burn group; -group 7: cells transfected with scramble control for miRNA-451 inhibitor and cultured with medium supplemented with 10% rat serum from burn group.
Zhou J., Lian H., Xu G, & Zhao T. (2020). MicroRNA-451 increases vascular permeability and suppresses angiogenesis in pulmonary burn injury in a rat model. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 29(11).
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