Hmao a and hmao b isozymes

The hMAO-A and hMAO-B isozymes, produced in BTI-TN-5B1-4 insect cells containing human cDNA, and their analyzed active units (U), were purchased from Sigma-Aldrich (St. Louis, MO, USA). Isozymes stocks were diluted with 1% of 1 M HEPES in Hank’s Balanced Salt Solution (HBSS) (pH 7.4) and aliquots stored at −80°C for single use. Standards of pirlindole, a reversible inhibitor for MAO-A (RIMA), deprenyl (DEP), an irreversible MAO-BI, and cell culture media and supplements were also purchased from Sigma-Aldrich. Different plant dry parts (leaves, stems, roots, petals, barks, resins, or whole herbs) were purchased from and identified by their trades companies including, East Earth Trade Winds (Redding, CA, USA), Mountain Rose, Herbs (Eugene, OR, USA), Mayway Corp. (Oakland, CA, USA), Monterey Bay Spice Comp. (Watsonville, CA, USA). The plants used were not specific to one region. Western blotting equipment and reagents were purchased from Bio-Rad Laboratories (Hercules, CA, USA) and BCA Protein Assay Kit from Peirce (Rockford, IL, USA). Amplex™ Red MAO Assay Kit was purchased from Molecular Probes by Life technologies™ (Eugene, OR, USA), and tyramine HCl from Santa Cruz Biotechnology (Dallas, TX, U.S.A.). MAO-Glo™ Kit was purchased from Promega Inc. (Madison, WI, USA).