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Human total mmp 3 elisa kits

Manufactured by R&D Systems
Sourced in United States

The Human Total MMP-3 ELISA kit is a quantitative sandwich enzyme immunoassay designed to measure the levels of total Matrix Metalloproteinase-3 (MMP-3) in human serum, plasma, and cell culture supernatants. The kit utilizes a solid-phase capture antibody and a horseradish peroxidase-conjugated detection antibody, resulting in a colorimetric reaction proportional to the amount of total MMP-3 present in the sample.

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2 protocols using human total mmp 3 elisa kits

1

Damiana Extract's Anti-Inflammatory Potential

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Damiana leaves powder was purchased from Ecuadorian Rainforest, LLC (Clifton, NJ, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin-streptomycin were supplied by Gibco RBL (Grand Island, NY, USA). Standard apigenin, positive control ascorbic acid, dexamethasone, and 5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Human Total MMP-1 and Human Total MMP-3 ELISA kits were purchased from R&D Systems (Minneapolis, MN, USA). Organic solvents were purchased from Samchun Chemical (Seoul, Korea) and Daejung Chemical & Metal (Siheung, Korea). Inorganic salts were purchased from Sigma-Aldrich. Silica gel was purchased from Merck (Kenilworth, NJ, USA). The primary and secondary antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA), Santa Cruz Biotechnology (Santa Cruz, CA, USA), and Bio-Rad Laboratories, Inc. (Hercules, CA, USA).
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2

Cytokine Regulation via Apigenin

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Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and penicillin-streptomycin were supplied by Gibco RBL (Grand Island, NY, USA). Standard apigenin, positive control ascorbic acid, dexamethasone, and 5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Human Total MMP-1 and Human Total MMP-3 ELISA kits were purchased from R&D Systems (Minneapolis, MN, USA). Organic solvents were purchased from Samchun Chemical (Seoul, Korea) and Daejung Chemical & Metal (Siheung, Korea). Inorganic salts were purchased from Sigma-Aldrich. Silica gel was purchased from Merck (Kenilworth, NJ, USA). The primary and secondary antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA), Santa Cruz Biotechnology (Santa Cruz, CA, USA), and Bio-Rad Laboratories, Inc. (Hercules, CA, USA).
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