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2 protocols using fenitrothion

1

Quantitative Analysis of Organophosphorus Pesticides

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The dopants, acetone and dichloromethane, were of analytical grade and purchased from Tianjin Kermel Chemical Reagent Co., Ltd. (Tianjin, China). Standard solutions of 1000 mg L−1 fenthion, imidan, dursban, dimethoate and malathion and 100 mg L−1 isocarbophos and fenitrothion were purchased from AccuStandard, Inc. (New Haven, CT, USA). Lower concentrations of the OPPs were obtained by the successive dilution of standard solutions with methanol.
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2

Pesticide Quantification and Method Development

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Pesticide standards (fenobucarb, ethoprop, diazinon, chlorothalonil, fenitrothion, methyl parathion, chlorpyrifos, thiobencarb, quinalphos, endosulfan I, endosulfan II, bifenthrin, fenpropathrin and permethrin) at 100 µg/mL and 1-chloro-3-nitrolbenzene (1000 µg/mL) used as internal standard with more than 95% purity, were purchased from AccuStandard Inc. New Haven CT, U.S.A. A working standard solution containing the pesticides was prepared daily by diluting the stock solution in methanol to a concentration of 10 µg/mL, and kept at 4 0 C before use. All solvents used were pesticide grade: methanol, acetone and acetonitrile were purchased from Fisher Scientific, Loughborough, U.K. Sodium chloride, ammonium chloride were purchased from Merck. The pH buffer solutions 4, 6, 8 -10 and 5 -7 were purchased from Fisher Scientific and Sigma-Aldrich respectively. Millipore filtered (0.45 µm) deionized water was used for method development.
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