The apoptotic cells were detected using Annexin V-allophycocyanin (Annexin V-APC) apoptosis detection kit (eBioscience, San Diego, CA, USA). The process of culturing infected cells in growth medium is the same as before. After trypsinization and PBS washes, the cells were washed with 1× binding buffer and centrifuged at 1300 rpm for 3 min. Then, the cell pellet was resuspended in 200 µL of 1× binding buffer, and Fadu cells were stained with 10 µL Annexin V-APC in the dark for 15 min and detected by ow cytometry using the FACS system.
Annexin 5 allophycocyanin annexin 5 apc apoptosis detection kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Annexin V-allophycocyanin (Annexin V-APC) apoptosis detection kit is a laboratory tool used to detect and quantify apoptosis, a programmed cell death process. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a molecule exposed on the surface of apoptotic cells. The Annexin V is conjugated with the fluorescent dye allophycocyanin, allowing for the identification and measurement of apoptotic cells using flow cytometry or fluorescence microscopy.
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Annexin V-APC Apoptosis Assay
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Annexin V-APC Apoptosis Assay
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The apoptotic cells were detected using Annexin V-allophycocyanin (Annexin V-APC) apoptosis detection kit (eBioscience, San Diego, CA, USA). The process of culturing infected cells in growth medium is the same as before. After trypsinization and PBS washes, the cells were washed with 1× binding buffer and centrifuged at 1300 rpm for 3 min. Then, the cell pellet was resuspended in 200 µL of 1× binding buffer, and Fadu cells were stained with 10 µL Annexin V-APC in the dark for 15 min and detected by ow cytometry using the FACS system.
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