L α phosphatidylethanolamine pe

The acylase from Aspergillus melleus (specific activity of 0.25 U mg⁻¹), Luria Bertani broth (LB)—for acylase QQ activity evaluation on C. violaceum, Mueller Hinton broth (MHB)—for determining minimal inhibitory/bactericidal concentrations, TSB—for biofilm inhibition tests and cetrimide selective agar for the culturing and enumeration of P. aeruginosa were purchased from Sigma-Aldrich, Madrid, Spain. The L-α-phosphatidylethanolamine (PE) was purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). The Live/Dead^® BacLight kit™ (Molecular probes L7012) and AlamarBlue™ reagent—for bacterial and human cells viability tests, respectively, were obtained from Invitrogen, Life Technologies Corporation (Sant Cugat del Vallès, Spain). The P. aeruginosa ATCC 10145, human foreskin fibroblasts (BJ-5ta cell line) and keratinocytes (HaCaT cell line) were obtained from the American Type Culture Collection (ATCC LGC Standards, Barcelona Spain). The Live/Dead^® kit for mammalian cells viability was obtained from Thermo Fischer Scientific (Sant Cugat del Vallès, Spain). All of the other reagents were purchased from Sigma-Aldrich (Spain), unless otherwise specified. Ultrapure MilliQ water with a resistivity of 18.2 MΩ cm was used in all of the experiments.

N-terminally formylated peptides (>95% purity) were purchased from Genscript Biotech, Rijswijk, The Netherlands. Hexafluoroisopropanol (HFIP), thioflavin T (ThT), lipopolysaccharide (LPS) from Gram-negative bacteria and trifluoroacetic acid (TFA) were purchased from Sigma Aldrich, St. Louis, MO, USA. Dimethyl sulfoxide (DMSO) was purchased from Merck, Darmstadt, Germany. 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DOPG), L-α-phosphatidylethanolamine (PE) extracted from E. coli, extrusion filters and filter supports were purchased from Avanti Polar Lipids, Birmingham, AL, USA.